Objective:Based on the method of network pharmacology and molecular docking,the molecular mechanism of the regulation of the active components of pueraria on sepsis encephalopathy was explored.Methods:The active components and potential targets of Radix Pueraria were obtained through Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP),Encyclopedia of Traditional Chinese Medicine(ETCM) and Comprehensive Database of Traditional Chinese Medicine(TCMID).Potential pathogenic targets of septic encephalopathy were identified through human Gene Database(GeneCards),online Mendelian database(OMIM),and DisGeNET database.The interaction network diagram of potential target genes for pueraria-sepsis encephalopathy was established using STRING database,and imported into Cytoscape software to screen out key target genes.Gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis were used to further explore the mechanism and therapeutic effect of pueraria regulating sepsis encephalopathy.Molecular docking technique was used to analyze the best effective components for docking pueraria with key target genes in sepsis encephalopathy.Results:Five active components and 106 target genes were screened from pueraria,of which 62 target genes crossed with the target genes of sepsis encephalopathy and were potential therapeutic targets.The active components of pueraria were thorns,β-sitosterol,puerariain,3'-methoxy soybeanoside,and soy oligition-4,7-alcohol glycoside showed better correlation with peroxisome profierator activated receptorγ,serine/threonine protein kinase 1,Cursin 3,matrix metal protease-9,and proto-oncogene(FOS),respectively.The signaling pathways of pueraria active ingredients for treating septic encephalopathy include lipid metabolism pathway,hepatitis B pathway,diabetes complication signaling pathway,relaxation signal pathway,and cell apoptosis pathway.Molecular docking results indicated that pueraria might be an effective ingredient for the treatment of sepsis encephalopathy.Conclusion:Active components of pueraria can regulate lipid metabolism by regulating core targets such as peroxisome profierator activated receptorγ,and play some roles for the treatment of septic encephalopathy.
维普
万方数据
