Objective:To explore the effect of catalpol-tetramethylpyrazine(CT) prescription on cognitive function in SAMP8 mice.Methods:Fifty 14-week-old SAMP8 mice were randomly divided into Model group,low dose CT prescription(CT-L) group,medium dose CT prescription(CT-M) group,high dose CT prescription(CT-H) group and donepezil hydrochloride(DH) group.Ten 14-week-old SAMR1 mice were chosen as Control group.The Control group and Model group were both gavaged with physiological saline,and each medication group was gavaged with corresponding drugs once a day for 8 consecutive weeks.Autonomous activity experiment,nesting behavior experiment,new object recognition experiment,and morris water maze experiment were adopted.After behavioral observations,superoxide dismutase(SOD),glutathione(GSH),β-amyloid protein(Aβ) 1-42,p-Tau,nuclear factor-κB(NF-κB) p65,nucleotide-bound oligomerized domain-like receptor protein 3(NLRP3),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and reactive oxygen species(ROS) levels were detected in the hippocampus of each group.The contents of NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS in serum of each group were detected.Results:Compared with Model group,the number of autonomous activities,nesting behavior scores,the time to distinguish new objects increased,the ratio of time to distinguish new,and old objects increased,the escape latency significantly shortened,the cross-platform number and residence time in the target quarter increased in DH group,CT-M group and CT-H group(P<0.05).Compared with CT-L group,the number of free activities,nesting behavior score increased,the identification time of exploring new objects significantly increased,the ratio of old and new object identification time increased,the escape latency shortened,the cross-platform number and the residence time in the target quarter increased in CT-M group,CT-H group,DH group(P<0.05).Compared with the CT-M group,the number of free activities,the score of nesting behavior,the identification time of exploring new objects,the ratio of old and new objects identification time,the escape latency period,the cross-platform number,and the target quadrant residence time of mice in the CT-H group increased(P<0.05).Compared with DH group,the number of free activities increased,nesting behavior score,identification time of exploring new objects increased,ratio of old and new object identification time increased,escape latency decreased significantly,cross-platform frequency,and target quadrant residence time increased in CT-H group(P<0.05).Compared with Model group,SOD and GSH levels in hippocampus of mice in DH group,CT-M group and CT-H group increased,while Aβ1-42,p-Tau,NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS levels decreased,the levels of serum NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS in mice decreased(P<0.05).Compared with CT-L group,the levels of SOD and GSH in hippocampus of mice in CT-M group,CT-H group and DH group increased,while the levels of Aβ1-42,p-Tau,NF-κB p65,NLRP3,TNF-α,IL-1β and ROS decreased,the levels of serum NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS in mice decreased(P<0.05).Compared with the CT-M group,the levels of SOD and GSH in the hippocampus of mice in the CT-H group increased,and the levels of Aβ1-42,p-Tau,NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS decreased,the levels of serum NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS in mice decreased(P<0.05).Compared with DH group,the levels of SOD and GSH in hippocampus of mice in CT-H group increased,and the levels of Aβ1-42,p-Tau,NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS decreased,the levels of serum NF-κB p65,NLRP3,TNF-α,IL-1β,and ROS in mice decreased(P<0.05).Conclusion:CT prescription could improve the imbalance between oxidation and reduction,inhibit inflammation in the brain,and thus improve cognitive impairment of SAMP8 mice,and the dose-effect relationship is positive.
Alzheimer's diseaseoxidative stresscatalpol-tetramethylpyrazine prescriptionneuroinflammationexperimental study
维普
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