Objective:To investigate the effect of sea cucumber saponin(SSC) on vascular inflammation in diabetic rats and its potential mechanism.Methods:SD rats were treated with high-sugar and high-fat diet,and streptozotocin ( STZ) intraperitoneal injection to construct diabetes model.The rats were divided into Control group,model group,metformin group(600 mg/kg),SSC group(30 mg/kg),and SSC+silencing information regulatory factor 1(SIRT1) inhibitor EX-527 group(SSC 30 mg/kg+EX-5275 mg/kg).After 4 weeks of drug intervention,fasting blood glucose(FBG) and fasting insulin(FINS) levels were detected,and insulin resistance index(HOMA-IR) was calculated.Serum vascular cell adhesion molecule-1(VCAM-1),monocyte chemokine-1(MCP-1),tumor necrosis factor α(TNF-α),and interleukin-1β(IL-1β) levels were detected by enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE) staining was used to observe the pathological changes of aorta.The expression of SIRT1/nuclear factor-κB(NF-κB) pathway-related proteins in aorta was detected by Western Blot.Results:Compared with Control group,the levels of FBG,FINS,HOMA-IR,serum VCAM-1,MCP-1,TNF-α,IL-1β,and nuclear NF-κB p65 protein in model group increased(P<0.05),the levels of body mass,aorta SIRT1 and nuclear factorκB inhibitory protein α(IκBα) decreased(P<0.05).Compared with model group,the levels of FBG,FINS,HOMA-IR,serum VCAM-1,MCP-1,TNF-α,IL-1β,and nuclear NF-κB p65 in SSC group and metformin group decreased(P<0.05),the levels of body mass,aorta SIRT1 and IκBα protein increased(P<0.05).Compared with SSC group,the levels of FBG,FINS,HOMA-IR,serum VCAM-1,MCP-1,TNF-α,IL-1β,and nuclear NF-κB p65 protein in SSC+EX-527 group increased(P<0.05),the levels of body mass,aortic SIRT1 and IκBα protein decreased (P<0.05).Conclusion:SSC can reduce vascular inflammation in diabetic rats,and its mechanism may be related to up-regulation of SIRT1 expression and inhibition of NF-κB activation.
diabetesvascular inflammationsea cucumber saponinsilencing information regulatory factor 1nuclear factor-κBexperimental study
维普
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