Potential Mechanism of Tongxinluo Capsules for the Treatment of Heart Failure
Objective:To explore the potential mechanism of Tongxinluo capsules for treating heart failure based on network pharmacology and molecular docking technology.Methods:The main traditional Chinese medicine components of Tongxinuo capsules were searched from Chinese Pharmacopoeia,and the corresponding active components and their targets were extracted using TCMSP database and BATMAN-TCM database system,and imported into Cytoscape 3. 8. 2 software to construct the "traditional Chinese medicine-active ingredient-target" diagram. Target data of "heart failure" were retrieved from GeneCards,OMIM and TTD databases,and the intersection was performed with the therapeutic target of Tongxinluo capsules.Protein interaction network diagram was constructed using STRING,and gene ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis were conducted using David database. The top 20 ranked pathways,active ingredients and targets were screened,and molecular docking were performed using DockThor website. Results:The active ingredients of Tongxinluo capsules included crocin,isoxanthopterin,D-mannitol,ursolic acid.It related through calcium ion signaling pathway,cyclic adenosine phosphate(cAMP) signaling pathway,interleukin-17(IL-17) signaling pathway,tumor necrosis factor(TNF) signaling pathway,C-type lectin receptor signaling pathway,acting on serine/threonine protein kinase B ( AKT1),recombinant protein A ( RELA),cyclooxygenase-2 ( PTGS2),mitogen-activated protein kinase 14(MAPK14),mitogen-activated protein kinase 1(MAPK1),and enhancer nuclear factor 1 of B cell κ light peptide gene(NFKB1) and other key targets for the treatment of heart failure.Conclusion:Tongxinluo capsules showed the advantages of multi-component,multi-target and multi-pathway synergism for the treatment of heart failure.
万方数据
维普
