Objective:To explore the mechanism of transcranial magnetic stimulation(TMS) on brain tissue injury in rats with middle cerebral artery occlusion(MCAO) based on myelocytic leukemia sequence 1(MCL-1).Methods:Thirty healthy free of specific pathogens (SPF) male SD rats(3 months old) were randomly divided into sham operation group,model group and TMS group,with 10 rats in each group.TMS treatment was performed within 24 h after successful modeling. Longa score was used to evaluate the degree of neurological impairment in 3 groups.The infarct area was measured by 2,3,5-triphenyltetrazolium chloride(TTC) staining.Apoptosis was detected by dUTP notch end labeling(TUNEL) mediated by terminal deoxynucleotide transferase.The protein expression of MCL-1 was detected by Western Blot. The mRNA expression of MCL-1 was detected by reverse transcription real-time fluorescent quantitative polymerase chain reaction(RT-PCR).Results:The nerve function deficit score and cerebral infarction area of rats in the sham operation group were less than those in the TMS group and model group,and the nerve function deficit score and cerebral infarction area of rats in the TMS group were less than those in the model group(P<0.05).The apoptosis index of the sham operation group was less than that of the TMS group and model group,and that of TMS group was less than that of model group(P<0.05).The expression of MCL-1mRNA in the sham operation group was more than that in the TMS group and model group,and the expression of MCL-1mRNA in the TMS group was more than that in the model group(P<0.05).The levels of MCL-1 protein in the sham operation group were higher than that in the TMS group and model group,and the level of MCL-1 protein in the TMS group was higher than that in the model group(P<0.05).Conclusion:TMS could reduce cell apoptosis and inhibit brain tissue injury in MCAO rats by increasing the expression of MCL-1mRNA.
middle cerebral artery occlusionbrain tissue injurymyelocytic leukemia sequence 1transcranial magnetic stimulationratsexperimental study
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