首页|紫外分光光度法测定微量人参木质素的含量

紫外分光光度法测定微量人参木质素的含量

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目的:建立微量人参木质素含量测定的紫外分光光度法.方法:采用经典的Klason法和紫外分光光度法.结果:紫外分光光度法测得的木质素含量较Klason法高,且重复性和精密度更高.人参木质素特征性吸收峰出现在260 nm波长处.紫外分光光度法的溴乙酰处理条件为70℃保温30 min.不同类型人参在木质素含量上有较大差异.结论:紫外分光光度法简便易行、快速准确,测定所需材料微量,适用于人参等名贵中药材的木质素含量测定.
Determination of Lignin Content in Tiny Panax ginseng by UV Spectrophotometry
Objective: To establish the UV spectrophotometry for determining lignin content in tiny Panax ginseng powder. Methods : Classical Klason method and UV spectrophotometry were used. Results: The lignin contents measured by UV spectrophotometry were higher, more repeatable and accurate as comparison with the Klason method. The specific absorptance peak of ginseng lignin appeared at 260nm. The acetyl bromide treatment of the UV spectrophotometry was processed for ginseng powder at 70℃ for 30 minutes. It is also concluded that the lignin contents were obvious different among various ginsengs. Conclusion; UV spectrophotometry is simple , accurate and just need a little materials. It is especially suitable to determine the lignin content for ginseng and other precious Chinese traditional medicines.

UV spectrophotometryLigninPanax ginseng

李靖、程舟、杨晓伶、李珊、顾敏、万树文、张文驹、陈家宽

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同济大学生命科学与技术学院,上海200092

上海雷允上药业有限公司,上海,200002

复旦大学生命科学学院生物多样性与生态工程教育部重点实验室,上海,200433

紫外分光光度法 木质素 人参

上海市科委资助项目

04DZ19834

2006

中药材
国家食品药品监督管理局,中药材信息中心站

中药材

CSTPCDCSCD北大核心
影响因子:0.913
ISSN:1001-4454
年,卷(期):2006.29(3)
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