首页|白豆蔻挥发油GC-MS指纹图谱研究

白豆蔻挥发油GC-MS指纹图谱研究

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目的:建立新的白豆蔻挥发油的GC-MS指纹图谱.方法:采用气相色谱-质谱(GC-MS)测定白豆蔻挥发油化学成分,得到GC-MS总离子流(TIC)指纹图谱,并用面积归一化法测定其相对含量;自编了提取多离子重建色谱(EMIC)软件,在GC-MS/TIC谱图中选择1,8-桉树脑、β-蒎烯、α-蒎烯、对伞花烯、α-乙酸松油醇酯等10个有代表性的特征成分,确定其特征离子,建立了10个特征成分的EMIC指纹图谱;同时研究了不同的提取方法,采用特殊的PR混合溶剂提取挥发油.结果:在GC-MS/TIC指纹图谱中鉴定出42种化合物,比色谱指纹图谱增加了定性信息;首次建立EMIC质量评价方法,并可计算出相对含量,更直观地、量化地评价白豆蔻及控制其中成药的加工、生产,以得到质量恒定的产品;PR溶剂可快速、简便地将白豆蔻挥发油提取出来.结论:本法简单快捷,效率高、成本低,得到的GC-MS/TIC指纹图全面完整,GC-MS/EMIC指纹图谱直观明确,为中草药深加工提供科学的质量控制方法.
Analysis of the Essential Olis from Amomun kravak Pierre ex Gagnep by GC-MS
Objective: To establish the fingerprint chromatogram of Amomun kravak Pierre ex Gagnep. Methods: The composition of the essential oil was isolated by PR solvent and determined by GC-MS. Forty-two species of compounds were identified, of which the relative contents were calculated using square peaks to normalization, among which the character components were 1,8-cineole, β-pi-nene, α-pinene, p-cymene, etc. EMIC software was used and EMIC fingerprint chromatogram was established by selecting the character ion of those 10 compounds. Different extraction methods were also compared. Results: The GC-MS fingerprint chromatogram can provide qualitative information confirming compounds of the corresponding chromatographic peaks. EMIC was first used to reason the quality control of the medicine quality and it can establish the foundation for safe and effective quality control of the medicine and its product. PR solvent can easily extract the essential oil from the medicine. Conclusion: This method has the advantage of high efficiency and low cost, and the GC-MS fingerprint chromatogram provided the ascription and quality of the medicine and will play an important role in promoting the modernization of Chinese traditional medicine.

Amomun kravak Pierre ex GagnepEssential oilGC-MSFinerprint

吴惠勤、黄晓兰、林晓珊、黄芳、葛发欢

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中国广州分析测试中心,广东省化学危害应急检测重点实验室,广东,广州,510070

中药提取分离过程现代化国家工程研究中心,广东,广州,510240

白豆蔻 挥发油 GC-MS 指纹图谱

广东省广州市科技攻关项目

2000J010001

2006

中药材
国家食品药品监督管理局,中药材信息中心站

中药材

CSTPCDCSCD北大核心
影响因子:0.913
ISSN:1001-4454
年,卷(期):2006.29(8)
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