Ginkgo biloba Extracts(EGb761) Inhibits Aflatoxin B1-induced Hepatocarcinogenesis in Wistar Rats
Objective: To investigate the effect of Ginkgo biloba extracts ( EGb761) on aflatoxin B1 (AFB;) -induced hepatocarcinogenesis and its antioxidant activity in Wistar rats. Methods: 71 Wistar rats were randomly divided into three groups; AFB1 (group A); AFB1 +EGb761( group B), Control (group C). Rats in gurop A and B were injected with AFB, through abdomen and the doses were 100-200 μg/kg, one to three times a week. Liver biopsy were performed in all rats during 14thw, 28thw, 42thw and 55thw, and were executed at 64thw. Gammaglutamyl transpeptidase-positive hyperplastic cell foci(7-GT foci) and histopathology of the liver tissue were observed. The levels of malondialdehyde (MDA) , as well as the activity of Glutathione peroxidase(GSH-Px) was examined. Results: At 42thw and 55thw, the γ-GT focus area( mm2/focus) and general area of foci (mm3/cm2) of group B were significantly smaller than that in group A (P = 0.000). The incidence of hepatocelluiar carcinoma (HCC) in group B (26. 92% ) was significantly lower than that in group A(76% ) (P =0. 000). Group C didnt have HCC development EGb 761 markedly increased GSH-Px activity, reduced MDA levels(P<0.05). Conclusion: EGB761 shows effective inhibition to hepatocarcinogenesis induced by AFB, in rats, which may be related to its antioxidant acctivity.
Extracts of Ginkgo biUa( EGb761)AFB1γ-GT fociHCCMDAGSH-Px
国家科技期刊平台
NSTL
万方数据
维普
