目的:研究雄黄微生物浸出液中无机砷抑制肿瘤S180的作用,并评价其毒性,为雄黄的二次开发提供参考依据.方法:建立小鼠S180腹水瘤模型,24 h后给药,1次/天,然后观察15 d的生命延长率;按照改良寇氏综合计算法,分析3种不同的给药方法(灌胃、腹腔、静脉)对小鼠的急性毒性.结果:矿物药雄黄浸出液中无机砷抑制肿瘤S180瘤株有明显的作用,并呈剂量依赖性,对S180腹水瘤的15 d 生存率达到10%～60%;3种给药方法的LD50值分别为5.27、3.63、2.68 mg/kg.结论:矿物药雄黄浸出液中无机砷具有抗肿瘤作用,其效果强于三氧化二砷注射液,但毒性强于三氧化二砷注射液.
The Inhibitory Effects of Realgax Bioleaching Solution on S180 Ascites-tumor and Its Toxicity
Objective: To investigate the inhibitory effect of Realgar bioleaching solution( RBS) on tumor S180 cells line and estimate its toxicity, to provide experimental evidence for the further exploit of Realgar. Methods ;24 hours after the models of ascites-tumor bearing mice were established, the mice were injected RBS once a day. The survival rates of S180 ascites-tumor bearing mice injected in RBS was studied, and the RBS acute toxicity of mice produced by oral, intraperitoneal or intravenous was evaluated by Drug Median Lethal Dose ( LD50). Results: The inhibitory effect of S180 cells in vivo had a dose-dependent manner. The survival rates of mice were 10% -60% in the different dose in 15 days. The LD50 values of RBS by oral, intraperitoneal or intravenous were 5. 27 mg/kg, 3.63 mg/kg and 2.68 mg/kg, respectively. Conclusion: RBS has potent antitumor effect, LD50 value of RBS is lower than that of traditional medicine.
国家科技期刊平台
NSTL
万方数据
维普
