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秦艽愈伤组织的培养及其龙胆苦苷含量的研究

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目的:为扩大和提高甘肃道地药材秦艽资源的利用效率.方法:以秦艽种子的萌发所获得的幼芽作为外植体,诱导形成愈伤组织;筛选诱导愈伤组织产生的不同培养基和培养条件,并继代培养;应用HPLC法测定龙胆苦苷的含量.结果:秦艽愈伤组织生长的最佳培养基为MS+2,4-D(1.0 mg/L) +6-BA(0.5 mg/L),最佳继代培养基为MS+2,4-D(1.0 mg/L) +6-BA(0.5 mg/L) +NAA(0.5 mg/L),最佳继代时间为30 d.龙胆苦苷积累量在指数生长后期(28 ~30 d)达最高,为0.78 mg/g DW.结论:筛选出了适宜的秦艽愈伤组织培养方法.秦艽愈伤组织在固体培养基上的生长曲线呈“S”型,且愈伤组织中龙胆苦苷的积累量随愈伤组织增殖而不断增加.
Callus Cultivation and Determination of Gentiopicroside from Gentiana macrophylla
Objective:To explore and improve the utilization efficiency of Gentiana macrophylla resources. Methods:The germination of Gentiona macrophylla seeds were used as explants to induce calluses; Cultured on various culture media and condition for callus induction and subculture by test; The content of gentiopicroside in callus was determined by HPLC. Results: The optimum medium for callus was MS + 2,4-D (1.0mg/L) +6-BA(0.5 mg/L) ,and MS+2,4-D(l. 0 mg/L) +6-BA(0.5 mg/L) +NAA(0.5 mg/L) was the optimum for subculture,the optimum time of subculture was 30 d; The accumulated gentiopicroside reached the highest level with 0.78 mg/g DW when entered 28 ~ 30 d. Conclusion:The optimized culture method of callus of Gentiana. macrophylla is obtained; The growth curve of callus of Gentiona macrophylla turns on "S" appearance and the accumulated gentiopicroside increases along with the callus appreciation.

Gentiana macrophylla Pall.CallusInductionGentiopicrosideHPLC

侯茜、郭美、张帆、周金霞、刘丽莎

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甘肃中医学院生物教研室,甘肃兰州730000

秦艽 愈伤组织 诱导 龙胆苦苷 HPLC

甘肃省中医药科研课题

07-GZK-1

2012

中药材
国家食品药品监督管理局,中药材信息中心站

中药材

CSTPCDCSCD北大核心
影响因子:0.913
ISSN:1001-4454
年,卷(期):2012.35(5)
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