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铁包金种子休眠及其破除方法初探

Primary Study on Dormancy and Release Method for Berchemia lineata Seed

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目的:探索铁包金种子休眠的原因及破除休眠的方法.方法:采用百粒法测定种子千粒重,TTC法测定种子生活力,吸胀法测定种子硬实率,对果实各部位的提取液进行发芽抑制物的生物鉴定;分别采用浓硫酸、热水、粗砂摩擦和赤霉素(GA3)处理来探究解除种子物理性和生理性休眠的最佳方法.结果:铁包金种子千粒重为10.82 9,硬实率高达100%,生活力为83%,其吸水速度和吸水率较低;浓硫酸处理10 min可提高其发芽率达85%,且显著提高其发芽势达41%;铁包金果实的不同部位均含发芽抑制物,其中种皮与种胚中发芽抑制物的抑制效果最强,500和1 000 mg/L GA3可显著提高种子的发芽势达48%和43%.结论:铁包金种子存在物理性和生理性休眠,致使种子萌发困难,这是导致铁包金资源减少的原因之一.
Objective:To explore the mechanism of the seed dormancy of Berchemia lineatea and the method of breaking the dormancy.Methods:Hundred-seed method,TTC and imbibition method was used to measure thousand seeds weight,seed viability and hard seed percentage,respectively.The germination inhibitor's biological characteristic was identified from the extract of every part in its fructification.Four treatments were compared to explore the best way to break physical and biological dormancy such as 98% sulfuric acid,hot water,grit friction and GA3.Results:The thousand seeds weight was 10.82 g,the percentage of hard seed was up to 100%,viability was 83%,its water absorption speed and absorption rate were relatively low.Being treated with 98% sulfuric acid for 10 minutes,the seed improved its germination rate to 85%,and significantly improved its germination potentiality to 41%.Every part of the fructification contained germination inhibitors.The strongest inhibitory effect was found in seed testa and embryo.500 and 1 000 mg/L GA3 significantly improved seed's germination potentiality to 48% and 43%.Conclusion:Berchemia lineata seed is hard to germinate because of its physical and biological dormancy,which is one reason for the resource reduction of Berchemia lineata.

Berchemia lineata (L.) DC.seedPhysical dormancyBiological dormancyRelease

叶燕莹、何梦玲、李东亮、熊洋、严寒静

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广东药学院中药学院,广东广州528200

铁包金种子 物理性休眠 生理性休眠 破除

广东省科技计划项目

2012B070300068

2013

中药材
国家食品药品监督管理局,中药材信息中心站

中药材

CSTPCDCSCD北大核心
影响因子:0.913
ISSN:1001-4454
年,卷(期):2013.36(9)
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