首页|HPLC测定铁包金不同部位中蒽醌类成分的含量

HPLC测定铁包金不同部位中蒽醌类成分的含量

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:建立HPLC同时测定铁包金不同部位中大黄素、大黄酚、大黄素甲醚的含量.方法:色谱柱为迪马ODS C1s(250 mm×4.6 mm,5μm);流动相为甲醇-0.2％磷酸溶液(74:26);流速:1.0 mL/min;柱温:35℃;检测波长:254 nm.结果:大黄素、大黄酚、大黄素甲醚分别在进样量为0.00201 ～0.0804 μg(r=0.9998),0.0066～0.264 μg(r=0.9997),0.0124 ～0.496(r=0.9998)范围内与峰面积线性关系良好;平均加样回收率分别为100.43％、101.29％、98.36％.铁包金根的总蒽醌含量高于藤茎.结论:该方法简便、重复性好,结果准确可靠,可用于铁包金药材的质量控制.
Determination of Anthraquinones from Different Medicinal Parts of Berchemia lineata by HPLC
Objective:To develop an HPLC method for determination of emodin,chrysophanol and physcion from different medicinal parts of Berchemia lineata.Methods:Samples were analyzed on Diamonsil ODS C 1s (250 mm × 4.6 mm,5 μm),with the mobile phase consisted of methanol-0.20％ phosphoric acid solution(74∶ 26).The flow rate was 1.0 mL/min,column temperature was set at 35 ℃,and detection UV wavelength was 254 nm.Results:The linear range of emodin,chrysophanol and physcion was 0.00201 ～ 0.0804 μg,0.0066 ～0.264 μg and 0.0124 ～0.496 μg,with the average recovery was 100.43％,101.29％ and 98.36％,respectively.The content of total anthraquinones in root was higher than that in taten of Berchemia lineata.Conclusion:The method is simple,accurate and reliable for quality control of Berchemia lineata.

Berchemia lineata (L.) DC.EmodinChrysophanolPhyscionAssayMedicinal part

王吉文、房志坚、成金乐、严寒静

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广东药学院,广东广州510006

中山市中智药业集团有限公司,广东中山528437

铁包金 大黄素 大黄酚 大黄素甲醚 含量测定 药用部位

2014

中药材
国家食品药品监督管理局,中药材信息中心站

中药材

CSTPCDCSCD北大核心
影响因子：0.913
ISSN：1001-4454
年,卷(期)：2014.37(6)
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