首页|白及多糖抗矽肺大鼠肺纤维化活性研究

白及多糖抗矽肺大鼠肺纤维化活性研究

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  • 国家科技期刊平台
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目的:研究白及多糖抗二氧化硅诱导的实验性矽肺大鼠肺纤维化活性及其可能的分子机理.方法:水提醇沉、Sevage法除蛋白制备白及多糖;二氧化硅气管滴注法建立大鼠矽肺模型;通过检测大鼠肺指数及组织病理学变化,结合血清SOD、MDA、NF-κB、IL-1β、PDGF、TGF-β1、TNF-α、HYP含量变化,流式细胞术检测CD3+、CD4+、CD8+T细胞百分含量及CD4+/CD8+比值,分析白及多糖药效及作用机理.结果:与模型组比较,白及多糖低(100mg/kg)、高(400 mg/kg)剂量组血清SOD活性显著升高,MDA、NO含量显著降低,CD4+/CD8+比值逆转(P＜0.01).除白及多糖高剂量能显著降低血清TNF-α含量外,白及多糖对矽肺大鼠血清炎症细胞因子及HYP水平均无明显影响.组织病理学检查显示,白及多糖未能有效改善肺组织纤维化程度.结论:白及多糖对矽肺大鼠机体抗氧化系统和免疫系统具有良好的调节作用,但不能有效抑制肺纤维化,白及中有效抑制肺纤维化活性组分有待进一步研究确证.
Research on the Anti-Pulmonary Fibrosis Effect of the Bletilla striata Polysaccharide in Rat Silicosis Model
Objective:To investigate the anti-pulmonary fibrosis effect and the possible molecular mechanism of the Bletilla striata polysaccharide.Methods:Polysaccharide was prepared by water reflux extraction plus ethanol precipitation method,and following deproteinization process by Sevage method.Rat silicosis model was established by invasive intratracheal instillation method.The effect and molecular mechanism of the polysaccharide was evaluated by lung indexes,lung pathological change,serum levels of SOD,MDA,NFκB,IL-1β,PDGF,TGF-β1,TNF-α,HYP were detected,and the contents of CD3 +,CD4 +,CD8 + T lymph cells and CD4 +/CD8 + ratio were detected by flow cytometry.Results:Both low (100 mg/kg) and high (400 mg/kg) dosage polysaccharide treatment could remarkably elevate the serum SOD level and reduce the MDA,NO level,and effectively reverse the CD4 +/CD8 + ratio comparing with the model group(P ＜0.01).Except the TNF-α level was significantly lower in the high dosage treatment group,there was no other effect in inflammatory cytokines and HYP content in serum.HE pathological section confirmed that the Bletilla striata polysaccharide treatment group can not effectively prevent lung fibrosis.Conclusion:The Bletilla striata polysaccharide has remarkable regulation effect on antioxidation system and immune system,but can not effectively prevent lung fibrosis,more effort should be made to study the active antipulmonary fibrosis components of Bletilla striata.

Bletilla striata (Thunb.) Reichb.f.SilicosisPloysaccharideAnti-oxidationImmunoregulation

李浩宇、史珍珍、舒立峰、王杰、李美芽、丁志山、蒋福升

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浙江中医药大学,浙江杭州310053

陕西省肿瘤医院,陕西西安710061

白及 矽肺 多糖 抗氧化 免疫调节

国家自然科学基金青年基金国家中医药管理局中药饮片质量保障体系研究浙江省自然科学基金浙江中医药大学校人才专项2014年浙江省大学生科技创新活动计划大学生科技创新项目

81503329201507002LY16H3100052014ZR122014R410044

2016

10.13863/j.issn1001-4454.2016.07.045

中药材
国家食品药品监督管理局,中药材信息中心站

中药材

CSTPCD北大核心
影响因子：0.913
ISSN：1001-4454
年,卷(期)：2016.39(7)
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