Effects of Different Drying Methods on Chemical Profile of Ophiopogon japonicus Root
Objective:Effects of different drying methods on the chemical profile of Ophiopogon japonicus root were compared,in order to screen optimum drying method and to provide the reference of standard processing method.Ophiopogon japonicus root was processed by traditional sun drying,drying using sulfur coal,drying using sulfur-free coal,and hot-air drying under different temperature (30,40,50,and 60 ℃).Moisture,total ash,water-soluble extracts contents and the constituents of seven homoisoflavones and saponins were selected for evaluating.Methods:Above constitutents were determined by UV-visible spectrophotometry and high performance liquid chromatography-quadrupole MS(HPLC-MS).Principal component analysis(PCA) and orthogonal partial least squared discriminant analysis(OPLS-DA) were used to distinguish the difference between Ophiopogon japonicus root which processed under different drying method.Results:All of the samples meet the Chinese Pharmacopoeia (edition 2015).Compared with the hot-air drying samples,the concentrations of homoisoflavones and saponins were lower in the traditional dried samples relatively.The drying methods had great impact on methylophiopogonone A,ophiopogonanone E,methylophiopogonanone A and methylophiopogonanone A.Conclusion:The 60 ℃ hot-air drying samples have a higher concentrations of homoisoflavones,saponins,and water-soluble extracte contents,whereas their total ash content is lower.Based on the obtained results,hot-air drying with 60 ℃ is the optimal method for the processing of Ophiopogonjaponicus root.
国家科技期刊平台
NSTL
万方数据
维普
