Identification of Medicinal Snakes and Water Extracts Based on DNA Mini-Barcode
Objective:To establish a method for identification of medicinal snake and its water extract by DNA mini-barcode.Meth-ods:By comparing the 16S rRNA sequences of medicinal snakes and 11 common adulterants,a mini-barcode of about 220 bp of snake DNA was established,and compared with the 16S rRNA standard barcode,the raw materials and water extracts of medicinal snakes were sequenced and identified.MEGA-X software was used to analyze genetic distance and construct phylogenetic tree by adjacency method(NJ).Results:The amplification rate and identification rate of DNA mini-barcode primers for medicinal snake raw materials and water extracts were 100％.The average intraspecific genetic distances of medicinal snakes and the adulterants were less than the average inter-specific genetic distances,All species had good monophyletic characteristics in the NJ phylogenetic tree,which was consistent with the results of 16S rRNA standard barcode raw material identification.Conclusion:The snake DNA mini-barcode established in this study can effectively realize the species identification of medicinal snake raw materials and water extracts,and provides technical support for ensu-ring the safety and effectiveness of snake raw materials,clinical decoction and formula granules.
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