Daphnane Diterpenoid Orthoesters of Miao Medicine Wikstroemia indica and the Antitumor Activities
Objective:To study the daphnane diterpenoid orthoesters of Miao Medicine Wikstroemia indica and the antitumor activi-ties.Methods:The daphnane diterpenoid orthoesters of Wikstroemia indica were isolated and purified by silica gel column chromatogra-phy,RP-C18 reversed phase column chromatography,Sephadex LH-20 glucan gel column chromatography and semi-preparative liquid chromatography,etc.The structures were identified by the physicochemical characteristics and spectral data.The cytotoxic activities of compounds 1,3,5 against hepatoma HepG2 cells line were evaluated by MTT method.Results:Five daphnane diterpenoid orthoesters were isolated from Miao medicine Wikstroemia indica and identified as 6α,7α-dihydyoxyhuratoxin(1),simplexin(2),12β-acetoxyhura-toxin(3),excoecafolin D(4),pimelea factor P2(5).These compounds showed the significant cytotoxicities against HepG2 cells.Conclu-sion:Among them,compound 1 is a new compound,compounds 2 to 5 are isolated from Wikstroemia indica for the first time.Compound 3 has significant effect on inhibition of HepG2 cell proliferation with IC50 value of(5.75±0.15)μg/mL.
国家科技期刊平台
NSTL
万方数据
