目的:建立坡柳皮HPLC指纹图谱,结合化学模式识别法对其进行质量评价,为坡柳皮药材质量控制及临床应用提供参考.方法:采用HPLC法建立坡柳皮指纹图谱;采用相似度评价、聚类分析、主成分分析和正交偏最小二乘判别分析等化学模式识别法对坡柳皮进行质量评价,同时测定异杞柳苷、(2R)-helichrysin A、(2S)-heli-chrysin A的含量.结果:建立的坡柳皮HPLC指纹图谱共标定12个共有峰,指认出4个成分;20批坡柳皮相似度均在0.990以上;聚类分析与主成分分析可将坡柳皮样品聚成2类;正交偏最小二乘判别分析筛选出异杞柳苷、(2R)-helichrysin A、(2S)-helichrysin A 3 个质量差异性成分,含量分别为 1.13％～3.25％、1.02％～3.23％、1.66％～6.45％.结论:采用指纹图谱结合化学模式识别技术可较全面地评价坡柳皮质量,该研究可为坡柳皮药材质量控制及临床应用提供参考.
Quality Evaluation Study of Salix myrtillacea Bark Based on Fingerprint Combined with Chemical Pattern Recognition
Objective:To establish the HPLC fingerprint of Salix myrtillacea bark and to evaluate the quality combine with chemi-cal pattern recognition method,so as to provide reference for quality control and clinical application of Salix myrtillacea bark.Methods:The fingerprint of Salix myrtillacea bark was established by HPLC.Chemical pattern recognition methods such as similarity evaluation,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used to evaluate the quality of Salix myrtillacea bark,and the contents of isosalipurposide,(2R)-helichrysin A,(2S)-helichrysin A were determined.Results:The HPLC fingerprint of Salix myrtillacea bark was established,a total of 12 common peaks were identified,four of the components were i-dentified.The similarity of 20 batches of Salix myrtillacea bark was above 0.900.By cluster analysis and principal component analysis,Salix myrtillacea bark samples were divided into two categories;Orthogonal partial least squares discriminant analysis was used to screen three quality difference markers,namely,isosalipurposide,(2R)-helichrysin A,(2S)-helichrysin A.The contents of above three compo-nents in 20 batches of samples were 1.13％to 3.25％,1.02％to 3.23％,1.66％to 6.45％,respectively.Conclusion:Using fingerprint combined with chemical pattern recognition technology can comprehensively evaluate the quality of Salix myrtillacea bark,which can provide reference for the quality evaluation and clinical application of Salix myrtillacea bark.
Bark of Salix myrtillacea Anderss.HPLCFingerprintChemical pattern recognition
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