Objective:To study the quality evaluation standard of Gypsophilae Radix,to establish the fingerprint analysis method.Methods:Water content,total ash content,acid insoluble ash content and ethanol soluble extract content of Gypsophilae Radix were de-termined according to the relevant methods in the appendix of Chinese Pharmacopoeia(2020 edition).HPLC method was used to deter-mine the content of index components in Gypsophilae Radix.The fingerprint of 30 batches of Gypsophilae Radix was established,and the quality was evaluated by similarity evaluation,cluster analysis,orthogonal partial least squares discriminant analysis(OPLS-DA).Re-sults:The mean values of water,total ash,acid insoluble ash,ethanol soluble extract,methyl gypsogenin 3-O-β-D-glucuronopyranoside,gypsogenin and quillaic acid were 8.89％,12.67％,0.79％,34.53％,13.58 mg/g,1.62 mg/g,1.03 mg/g,respectively.A total of 17 common peaks were found in 30 batches of Gypsophilae Radix fingerprint.The similarity of 30 batches of samples ranged from 0.916 to 0.997.The samples were divided into three categories by cluster analysis,and seven different components were screened out by OPLS-DA analysis.Conclusion:The method established in this study can provide reference for the establishment of quality standard of Gyp-sophilae Radix.
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