Study on the Fingerprint and Spectral Effect Relationship of Aboveground Parts of Laggera pterodonta Based on Electronic Nose Technology
Objective:To establish the odor fingerprint of 15 batches of the aboveground parts of Laggera pterodonta by ultra-fast gas phase electronic nose technology,to screen the components related to antioxidant activity by combining with spectral effect relation-ship analysis.Methods:The ultra-fast gas phase electronic nose technology was used to establish the odor fingerprint of 15 batches of the aboveground parts of Laggera pterodonta.Similarity evaluation was conducted and qualitative analysis was finished with the AroChem-Base database.The antioxidant activity of the aboveground parts of Laggera pterodonta was evaluated using DPPH and ABTS free radical scavenging ability.Multivariate statistical analysis was used to evaluate the spectral effect relationship and the components with high cor-relation with antioxidant activity were screened out.Results:The odor fingerprint of 15 batches of the aboveground parts of Laggera pter-odonta was established,and 14 common peaks were demarcated with AroChemBase database,which were 2-methylbutane,5-ethylidene-2-norbornene,a-pinene,a-phellandrene,1,8-cineole,5-methyl-4-nonene,limonene,1,2-dibromo-3-chloropropane,linalool,methyl euge-nol,methyl undecanoate,β-caryophyllene,undecyl acetate and rheosmin.The IC50 of DPPH and ABTS free radical scavenging activity of the volatile components of the aboveground parts of Laggera pterodonta was 6.56 to 21.26 mg/mL,3.89 to 18.34 mg/mL,respectively.The results of grey correlation analysis and partial least squares regression analysis showed that the limonene,methyl eugenol,rheosmin and others had significant correlation with antioxidant activity.Conclusion:The odor fingerprint established by ultra-fast gas phase elec-tronic nose connected with analysis of spectral effect relationship can screen out three volatile components with significant correlation to antioxidant activity.This study provides a basis for the quality control of the aboveground parts of Laggera pterodonta.
Aboveground parts of Laggera pterodonta(DC.)Benth.Ultra-fast gas phase electronic noseAntioxidantionFinger-printSpectral effect relationship
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