Effects of Piperlongumine on Apoptosis of Breast Cancer and Breast Cells via STAT3/HIF-1α Pathway
Objective:To investigate the effect of piperlongumine on the proliferation,apoptosis and cell cycle of breast cancer cells MDA-MB-231,MCF-7 and normal breast cells MCF-10A and its potential molecular mechanism.Methods:MDA-MB-231,MCF-7 and MCF-10A cells were treated with different concentrations of piperlongumine,and the cell proliferation was detected by MTT assay.Cell clone formation assay was used to detect cell clone formation ability.Flow cytometry was used to detect cell apoptosis and cell cycle.The protein expressions of cleaved Caspase-3,Bcl-2,Bax,Cyclin D1,p53,p-JAK2,p-STAT3,HIF-1α and Survivin were detected by Western Blot.Results:Piperlongumine inhibited the proliferation and induced apoptosis of MDA-MB-231 and MCF-7 cells in a dose-dependent manner,but had no obvious inhibitory effect on MCF-10A cells.The protein expressions of p53,Bcl-2,Cyclin D1,p-STAT3,Survivin,HIF-1α in MDA-MB-231 cells and p53,p-STAT3,Survivin,HIF-1α in MCF-7 cells were down-regulated and cleaved Caspase-3 and Bax protein expressions in MDA-MB-231 cells were up-regulated.Conclusion:Piperlongumine can inhibit the proliferation and induce apoptosis of breast cancer cells MDA-MB-231 and MCF-7,the mechanism may be related to the negative regulation of STAT3/HIF-1α pathway.
国家科技期刊平台
NSTL
万方数据
