摘要
目的 运用网络药理学探讨小儿止哮平喘颗粒治疗支气管哮喘的作用机制.方法 通过TCMSP和SymMap数据库筛选小儿止哮平喘颗粒的活性成分及其作用靶点,通过NCBI Gene、GeneCards、TTD、KEGG、MalaCards、PharmGKB、OMIM等数据库筛选支气管哮喘的疾病相关靶点,获取活性成分作用靶点与疾病相关靶点的交集靶点(共同靶点),即小儿止哮平喘颗粒治疗支气管哮喘的潜在作用靶点.运用Cyto-scape3.2.1软件构建"中药-成分-靶点-疾病"网络,将上述共同靶点导入String数据库,获取数据后在Cyto-scape3.2.1软件中构建"成分靶点-疾病靶点"(T-D)网络,利用Metascape数据库进行GO和KEGG通路富集分析.结果 共筛选得到289个活性成分及470个潜在作用靶点;获得豆甾-4-烯-3-酮、白矢车菊素、丹参醌酚I、飞燕草素、(E)-(4-甲基亚苄基)-(4-苯基三唑-1-基)胺、油酸等核心活性成分;RAC-a丝氨酸/苏氨酸蛋白激酶(RAC-alpha serine/threonine-protein kinase,AKT1)、p53 蛋白(cellular tumor antigen p53,TP53)、黏着连接蛋白β1(catenin β1,CTNNβ1)、转录因子激活蛋白-1(transcription factor AP-1,JUN)、Harvery鼠肉瘤病毒癌基因(GTPase HRas,HRAS)、生长因子受体结合蛋白2(growth factor receptor-bound protein 2,GRB2)、过氧化物酶体增殖激活受体 γ(peroxisome proliferator-activated receptor gamma,PPARG)等核心靶点;筛选得到AGE-RAGE信号通路、钙信号通路、cAMP信号通路、TNF信号通路、PI3K-Akt信号通路等关键信号通路.结论 小儿止哮平喘颗粒可通过多成分、多靶点调控多种通路达到治疗小儿支气管哮喘的作用,为其分子机制研究奠定了一定的基础.
Abstract
Objective To explore the mechanism of Xiaoer Zhixiao Pingchuan Granule(小儿止哮平喘颗粒)in the treatment of bronchial asthma by means of network pharmacology.Methods The active components and ac-tion targets of Xiaoer Zhixiao Pingchuan Granule were screened by TCMSP and SymMap database,and the disease-related targets of bronchial asthma were screened by NCBIGene,GeneCards,TTD,KEGG,MalaCards,PharmGKB,OMIM and other databases,and the intersection targets(common targets)of active components and disease-related targets were obtained,that is,the potential targets of Xiaoer Zhixiao Pingchuan Granule in the treatment of bronchial asthma.The"traditional Chinese medicine-chemical composition target-disease"network was constructed by Cyto-scape3.2.1 software,and the above common targets were imported into String database to obtain data.The"compo-nent target-disease target"network was constructed in Cytoscape3.2.1 software,and GO and KEGG pathway enrich-ment analysis was carried out by using Metascape database.Results A total of 289 active components and 470 po-tential targets were screened,and the core active components such as ethycholest-4-en-3-one,(+)-Leucocyanidin,miltionone I,delphinin,(E)-(4-methylbenzylidene-(4-phenyltriazol-1-yl)amine,oleic acid),AKT1,TP53,CTNNβ1,JUN,HRAS,GRB2 and PPARG were obtained.AGE-RAGE signal pathway,calcium signal pathway,cAMP signal pathway,TNF signal pathway,PI3K-Akt signal pathway and other key signal pathways were screened.Conclusion Xiaoer Zhixiao Pingchuan Granule can treat bronchial asthma by regulating multiple pathways with multi-components and multi-targets,which lays a foundation for the study of its molecular mechanism.
基金项目
中国中医科学院科技创新工程重大攻关项目(CI2021A04701)
中国中医科学院科技创新工程重大攻关项目(CI2021A02505)
北京市级中医药专家学术经验继承工作指导项目(第六批)(京中医科字[2022]80号)
国家科技期刊平台
NSTL
万方数据