Objective To investigate the possibility of establishing a rat model of spasmodic cough after myco-plasma pneumoniae pneumoniae(MPP)infection by using"mycoplasma pneumoniae culture solution intranasal in-stillation+smoking exposure+capsaicin solution nebulisation".Methods 40 SPF-grade male Wistar rats were ran-domly divided into four groups:control group,MPP group,smoking exposure group,and MPP spasmodic cough group.The MPP spasmodic cough rat model was established by using the combined approach of"mycoplasma pneu-moniae culture solution intranasal instillation+smoking exposure+capsaicin solution nebulization".General behavior-al manifestations,cough frequency,total white blood cell count in bronchoalveolar lavage fluid(BALF),and patho-logical changes in lung tissue were observed and recorded in each group.Results During the experiment,the con-trol group rats exhibited normal behavioral manifestations.With increasing days of smoking exposure,rats in the mod-el groups showed symptoms such as lethargy,hoarse breathing,and intermittent cough in some individuals.After capsa-icin solution nebulization,rats in all model groups exhibited obvious cough symptoms,characterized by neck exten-sion,mouth opening,and ear tremor.Compared with the control group,the MPP spasmodic cough group had a signifi-cantly increased cough frequency,with a statistically significant difference(P＜0.05).The MPP group and smoking ex-posure group showed an increased cough frequency,but the difference was not statistically significant(P＞0.05).Com-pared with the control group,the total white blood cell count in BALF was significantly increased in the MPP group,smoking exposure group,and MPP spasmodic cough group,with a statistically significant difference(P＜0.05).Howev-er,there was no significant difference between the three model groups(P＞0.05).Conclusion It is feasible to estab-lish a rat model of spasmodic cough after mycoplasma pneumoniae pneumoniae(MPP)infection by using"mycoplas-ma pneumoniae culture solution intranasal instillation+smoking exposure+capsaicin solution nebulisation".
mycoplasma pneumoniae pneumoniaspasmodic coughrat modelexperimental study
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