Exploring the molecular mechanism of related Chinese medicines acting on key genes in autism based on network pharmacology
Objective To screen the key genes of autism based on network pharmacology,and explore the po-tential targets,biological processes and signaling pathways related to traditional Chinese medicines in autism.Meth-ods GSE29691 dataset was selected from GEO database as research sample.The differential genes were screened based on GEO2R,the differential genes were analyzed by GO and KEGG using Wei Sheng Xin network tool,key tar-gets were screened and modules were built using Cytoscape software,key targets were imported into Coremine medi-cal database for visual analysis,and potential Chinese medicines were screened.Results The original dataset from GSE29691 was processed to screen out 164 genes that were significantly different from those in autistic patients.GO and KEGG analysis showed that differential genes were mainly involved in the regulation of EPK1 and EPK2 protein linkage,positive regulation of T cell activation and leukocyte mediated cytotoxicity,and were associated with salmo-nella infection pathways and diabetic cardiomyopathy.The PPI network map showed that the regulatory granolysin(GNLY),T cell regulatory chemokine(CCL5),Lysosome associated membrane protein 1(LAMP1),natural killer cell granuloprotein 7(NKG7),T cell surface single pass membrane glycoprotein(CD3E),cysteine protease inhibi-tor(CST7),perforin 1 recombinant protein(PRF1),histoprotease(CTSW),fibroblast growth factor binding pro-tein(FGFBP2),etc as a potential target for treating autism.Cordycepssinensis,silkworm sand and silkworm moth are related to several key genes of autism,and silkworm sand and silkworm moth are related to CCL5,CANX,LAMP1 and other key genes.Cordyceps sinensis and GNLY,CCL5,LAMP1 and other key genes map each other;In addition,active ingredients such as Cordycepssinensis and silkworm sand indirectly participate in key autism genes such as GNLY,CCL5,lysosomal associated membrane protein 1(LAMP1)and calponectin(CANX)through TNF and TLR4,which may become potential target Chinese medicine for the treatment of autism.Conclusion The active compounds in Cordycepssinensis,silkworm moth and silkworm sand can regulate inflammation and immune system by acting on multiple targets such as CCL5,LAMP1 and CANX,and play a therapeutic role in autism.
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