Study on comparative of polysaccharide extraction processes from Boletus aereus and their in vitro antioxidant activity
Using the yield as the standard,the optimal extraction methods for polysaccharides from Boletus aereus was screened and optimized among three extraction methods(hot water extraction,ultrasound extraction,and ultrasound-assisted composite enzyme method).Subsequently,the in vitro antioxidant activity of B.aereus polysaccharides was investigated.A single-factor assay was used to select the method with the highest polysaccharide yield.The response surface methodology was employed to further optimizing.Polysac-charides were graded using a stepwise alcohol precipitation method.The in vitro antioxidant activity was evaluated by DPPH assay,ABTS assay,hydroxyl free radical scavenging assay,and reducing power assay.The polysaccharide yields of hot water extraction,ultrasound ex-traction,and ultrasound-assisted composite enzyme extraction were 5.64％,6.74％,and 18.47％,respectively.The optimized process for ultrasound-assisted composite enzyme extraction was as follows:enzyme ratio(cellulase:protease)of 2:1,enzymatic hydrolysis temperature of 42.7 ℃,enzymatic hydrolysis time of 67 min,enzyme addition of 1.92％,ultrasound time of 20 min,and an expected yield of 18.867％.The B.aereus polysaccharides obtained exhibited excellent antioxidant activity.Their antioxidant capacities were pos-itively correlated with polysaccharide concentration and alcohol precipitation concentration.The polysaccharides obtained by 80％etha-nol precipitation showed the best overall antioxidant activity in vitro.Ultrasound-assisted composite enzyme extraction significantly im-proves the polysaccharide yield of B.aereus.The obtained polysaccharides exhibit excellent in vitro antioxidant activity.
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维普
