Prescription regularity and mechanisms of TCM medicine in treatment of granulomatous lobular mastitis:a study based on data mining and network pharmacology
Objective:To explore the medication rule and potential mechanism of TCM in the treatment of granulomatous lobular mastitis,in order to provide reference for follow-up experimental research and clinical application.Methods:Retrieve the Chinese literature on the treatment of granulomatous lobular mastitis with TCM medicine included in the database of CNKI,Wanfang and VIP,establish the relevant prescription database,and use Excel,SPSS,OriginPro and Cytascape to carry out frequency statistics,drug efficacy classification,sex and taste analysis,association rules,and cluster analysis of the TCM medicine in the prescription.The network pharmacology method was used to detect the potential target and pathway of the core drug,the target information related to the drug and granulomatous lobular mastitis from TCMSP was obtained,Disgene and other databases,upload the intersection target to STRING database for PPI analysis,Cytascape 3.9.1 was imported to draw the PPI network diagram and conduct network topology analysis to obtain the core target.Finally,the core components and core targets are verified by molecular docking.Results:A total of 63 eligible literature articles were included,involving 162 flavors of TCM medicine,among which Zaojiaoci(Spina Gleditsiae),Gancao(Radix Glycyrrhizae),Chaihu(Radix Bupleuri),Pugongying(Herba Taraxaci),Danggui(Radix Angelicae Sinensis)etc.were used the most.The natures and taste of the drug is mainly warm,cold and bitter,sweet with spicy,mainly attributed to the liver,lungs,stomach and spleen.A total of 10 drug pairs and 9 drug groups were obtained in the association rule analysis,among which Danggui-Huangqi(Radix Astragali)appeared most frequently.Cluster analysis yielded 7 clustering squares.There were 16 intersection targets of the core drug combination Danggui,Huangqi,Zaojiaoci,Jinyinhua(Flos Lonicerae Japonicae),Pugongying and GLM,and 9 key targets were obtained by topological analysis,including ALB,STAT3,IL-6,etc..The DAVID platform was used to perform GO function enrichment analysis and KEGG signaling pathway enrichment analysis,mainly involving HIF-1 signaling pathway,Toll-like receptor signaling pathway,TNF signaling pathway.The molecular docking results showed that the core components were well connected to the target,and luteolin had the best binding to ALB.Conclusion:The main treatment for granuloma lobular mastitis is to nourish Qi(气)and blood,and support sore and muscle.The prescription mainly consists of tonifying deficiency drugs,supplemented by heat clearing drugs.The core drug group exerts therapeutic effects by regulating the HIF-1 signaling pathway,Toll like receptor signaling pathway,TNF signaling pathway,etc..
Data miningGranulomatous lobular mastitisNetwork pharmacologyMolecular dockingMastitis
万方数据
维普
