Discussion on the potential mechanism of the Linggui Zhugan decoction in improving non-alcoholic fatty liver disease based on network pharmacology and molecular docking technology
Objective:To predict and explore the active ingredients and the potential action mechanism of the Linggui Zhugan decoction(苓桂术甘汤)ameliorating non-alcoholic fatty liver disease by network pharmacology and molecular docking technology.Methods:The active ingredients and related potential targets of the Linggui Zhugan decoction were screened out by TCMSP,ETCM database.Related targets of non-alcoholic fatty liver disease were retrieved by GeneCards database.Based on the matching results of the potential targets of non-alcoholic fatty liver disease and the Linggui Zhugan decoction,core targets were screened by protein interaction analysis in the STRING platform.Active ingredient-non-alcoholic fatty liver disease-target-pathw network was built by Cytoscape 3.9.1 software.Reactome and Metascape databases were used for GO and KEGG enrichment analysis.Autodock Tools 1.5.7 was used to conduct molecular docking between the first 5 core targets and the corresponding active ingredients.Results:The core active components of the Linggui Zhugan decoction on non-alcoholic fatty liver disease were quercetin,naringenin,kaempferol,etc..The core targets were TP53,TNF,AKT1,IL-1β and IL-6.It mainly involves biological processes such as cellular responses to lipids,response to xenostimuli,response to inorganic substances They were mainly enriched in cancer pathway,AGE-RAGE signaling pathway in diabetic complications,IL-17 and other signaling pathways.TP53,TNF,IL-6 and IL-1β were docked well with quercetin,and AKT1 was docked well with naringenin.Conclusion:The Linggui Zhugan decoction treated non-alcoholic fatty liver disease in multiple directions and multiple dimensions.This study provides theoretical basis and reference for further revealing the mechanism of the Linggui Zhugan decoction on non-alcoholic fatty liver disease.
万方数据
维普
