Effects of the Jianpi Qutan prescription on body composition/behavioral changes in ApoE-/-mice with restricted activities
Objective:To observe the effect of the Jianpi Qutan prescription(健脾祛痰方)on the changes of body composition/behavior in ApoE-/-mice with restricted activities.Methods:A total of 30 ApoE-/-mice were randomly divided into the model group,the Atto vastatin group,the low concentration group,the middle concentration group and the high concentration group of the Jianpi Qutan prescription,and 6 C57BL/6J mice were taken as the normal group.The normal group was fed with normal diet and the other 5 groups were fed with high fat diet.The Atto vastatin group was fed with Atto vastatin 2.4 mg/(kg·d).The low concentration group,the middle concentration group and the high concentration group were fed with the Jianpi Qutan prescription[2.97 g/(kg·d),5.94 g/(kg·d),11.88 g/(kg·d)],and the normal group and the model group were fed with the same volume of normal saline.The model of spleen deficiency and phlegm turbidity was evaluated after 12 weeks.During the experiment,the fur color,activity ability,stool characteristics and body composition of mice were observed and recorded every week,and the open field test and treadmill test were used to evaluate the animal behavior.Results:Compared with the normal group,the total exercise distance of mice in the model group was reduced in the open field test,the exhaustion time of mice in the treadmill test and the body fat of mice in the model group were decreased,with statistical significance(P<0.01).Compared with the model group,the total exercise distance of mice in the low concentration group,the middle concentration group and the high concentration group of the Jianpi Qutan prescription in open field test showed an increasing trend,but the difference was not statistically significant(P>0.05).The running time of mice in the middle concentration group and the high concentration group of the Jianpi Qutan prescription was increased in treadmill test,and the difference was statistically significant(P<0.05,P<0.01).The running time of the Atto vastatin group and the low concentration group of the Jianpi Qutan prescription showed an increasing trend,but there was no statistical significance(P>0.05).Body composition test of the Jianpi Qutan prescription showed an increasing trend of body fat in the low concentration group,the middle concentration group and the high concentration group of the Jianpi Qutan prescription,but there was no statistical significance(P>0.05).The body fat in the Atto vastatin group was significantly increased,and the difference was statistically significant(P<0.01).Conclusion:The Jianpi Qutan prescription can increase the behavioral activity of ApoE-/-mice with restricted activity,but has no significant effect on body composition.
The Jianpi Qutan prescriptionAtherosclerosisAnimal behaviorBody fatApoE-/-mice
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