A study on the network pharmacological mechanism of the Shaoyao Gancao decoction in the treatment of cervical spondylosis
Objective:To explore the network pharmacological mechanism of the Shaoyao Gancao decoction(芍药甘草汤),a commonly used prescription in TCM on cervical spondylosis.Methods:The main active ingredients of the Shaoyao Gancao decoction[Shaoyao(Radix Paeoniae Alba),Gancao(Radix Glycyrrhizae)]were selected by BATMAN-TCM and their targets were obtained after comparison by the protein database Uniprot.The targets of cervical spondylosis were obtained from the disease databases OMIM,GeneCards and related literature.The intersection of the Shaoyao Gancao decoction and cervical spondylosis was sorted out and collected to obtain common targets.The protein-protein interaction network diagram was constructed from the obtained common targets through the protein interaction network database STRING.Then,the biological processes and signaling pathways that may be related to the treatment of cervical spondylosis were analyzed by GO and KEGG enrichment analyses using the bioinformatics database DAVID platform.Results:(1)A total of 79 active ingredients and 442 potential targets of the Shaoyao Gancao decoction were screened.There were 765 disease targets and 45 drug-disease targets.(2)GO analysis showed that the main biological process of the Shaoyao Gancao decoction in the treatment of cervical spondylosis might be through the regulation of Akt serine/threonine protein kinase 1,interleukin-6,epithelial growth factor,estrogen receptor 1 and other targets,and was related to protein and DNA-binding enzymes,and also involves lipopolysaccharide reaction,negative regulation of apoptosis and differentiation,cell response to organic cyclic compounds and other biological processes.(3)KEGG enrichment analysis showed that the main pathways of the Shaoyao Gancao decoction in the treatment of cervical spondylosis were related to multiple signaling pathways such as mitogen-activated protein kinase,nuclear factor-κB,and tumor necrosis factor.Conclusion:It is speculated that the Shaoyao Gancao decoction mainly regulates mitogen-activated protein kinase,nuclear factor,tumor necrosis factor and other signaling pathways by regulating Akt serine/threonine protein kinase 1,interleukin-6,epithelial growth factor,estrogen receptor 1 and other targets,and then intervenes in the anti-apoptosis,adhesion,extracellular matrix degradation of nucleus pulposus,vascular growth,inflammation and catabolic mediator levels in cervical spondylosis.
The Shaoyao Gancao decoctionCervical spondylosisNetwork pharmacology
NETL
NSTL
万方数据
