A study on the regularity and molecular mechanism of treating chronic pharyngitis with the Dangshen containing prescription based on data mining and network pharmacology
Objective:To explore the regularity and molecular mechanism of the Dangshen(Radix Codonopsis)containing prescription in treating chronic pharyngitis,and to provide reference for the clinical application and new drug research development of Dangshen.Methods:Based on the method of data mining,SPSS Modeler and Python 3.7 software were used to analyze the flavor and meridian tropism,common drug compatibility and prescription rules of the prescription containing Dangshen in the treatment of chronic pharyngitis.Then,network pharmacology method was used to analyze the molecular mechanism of Dangshen in the treatment of chronic pharyngitis,and Cytoscape software was used for visualization.In this study,software such as AutoDock and PyMOL were used for molecular docking.Finally,the stability of the protein-ligand complex was verified by computer molecular simulation.Results:A total of 125 prescriptions containing Dangshen were obtained for treating chronic pharyngitis,and 21 high-frequency drugs were found.Forty high-frequency drug combinations were obtained by association rules,four groups of drug cluster combinations were obtained by cluster analysis,and 11 common factors were extracted by factor analysis.A total of 31 chemical components and 38 drug-disease intersection genes were screened by network pharmacology,and 65 pathways were obtained by KEGG enrichment analysis.Molecular docking results showed that CASP3 had the strongest binding activity with stigmasterol.Conclusion:Dangshen plays a therapeutic part in diabetes through the regulation of core targets,such as EGFR,CASP3,CCND1,VEGFA and through intervention in Epstein-Barr virus infection,HIF-1 signaling pathway,TNF signaling pathway,PI3K-Akt signaling pathway,and other signaling pathways,which provide a basis for further study and the resource utilization of Dangshen.
万方数据
维普
