Detection of main components of Sanhuang Ointment based on UPLC-MS/MS
Objective:To analyze and identify the chemical components of Sanhuang Ointment(三黄膏)by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods:A total of 50 mg of Sanhuang Ointment was taken and added into methanol intemal standard extract.After swirling,supematant was taken and filtered by microporous filter membrane for detection.Liquid phase conditions:Agilent SB-C18 1.8 μm,2.1 mm×100 mm column with ultra-pure water(with 0.1％formic acid)and acetonitrile(with 0.1％formic acid)as mobile phases,gradient elution was performed,flow rate was 0.36 mL/min,column temperature was 40℃,injection volume was 2 μL.Mass spectrometry conditions:electrospray ionization(ESD temperature was 500℃.Ion spray voltage was 5 500 V(positive ion mode)/-4500 V(negative ion mode).Ion source gas Ⅰ(GSD,gas Ⅱ(GSⅡ)and curtain gas were set to 50,60 and 25 psi,respectively,and collision-induced ionization parameters were set to high.QQQ scanning was performed using MRM mode and the collision gas(nitrogen)was set to medium.Further declustering potential(DP)and collision energy(CE)optimization was accomplished for DP and CE of individual MRM ion pairs.A specific set of MRM ion pairs was monitored in each period depending on the metabolites eluted within each period.Data was collected in positive and negative ion mode by electrospray ion source.Results:A total of 30 compounds were identified and postulated,including 11 alkaloids,11 flavonoids,3 terpenoids,and 5 phenolic acids.Conclusion:Comparison of the 30 compounds identified by UPLC-MS/MS with the main compounds of Huangqin(Scutellaria baicalensis),Huanglian(Radix Scutellariae),and Huangbo(Radix Phellodendron)reveals that the processed Sanhuang Ointment retains the main chemical components of Huangqin,Huanglian,and Huangbo.It lays the foundation for future research on the material basis of Sanhuang Ointment.
Sanhuang OintmentUltra high performance liquid chromatography-tandem mass spectrometryChemical composition spectrum
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