摘要
目的:本研究采用高效液相色谱法(High Performance Liquid Chromatography,HPLC)建立了蒲公英药材中绿原酸、咖啡酸、木犀草苷、菊苣酸、木犀草素5种成分的含量测定方法,完善和提高蒲公英的质量控制方法.方法:蒲公英药材样品经过超声法提取,分离与检测采用Waters ACQUITY BEH C18色谱柱(2.1 mm× 100mm,1.8 μm),以乙腈(B)-0.4％磷酸水溶液(A)为流动相,梯度洗脱;检测波长为320nm;流速:1.0mL/min;进样量:20μL;柱温:35 ℃.通过专属性考察、线性关系考察、精密度考察、重复性考察、稳定性考察和加样回收率试验来验证方法学.结果:绿原酸、咖啡酸、木犀草苷、菊苣酸和木犀草素的质量浓度范围分别为 60～1 920 μg/mL、3.22～103 μg/mL、3.22～103 μg/mL、68.125～2 180 μg/mL 和 3.31～106 μg/mL,以各成分的质量浓度(X)为横坐标、峰面积(Y)为纵坐标进行线性回归分析,呈良好的线性关系(r＞0.998 7),平均加样回收率为98.62％～101.43％,RSD为0.68％～1.86％,仪器精密度、方法重复性和方法的稳定性良好(RSD均＜2％).结论:该方法分离度好,精密度、稳定性、重复性均良好,加样回收率高,可作为蒲公英的质量控制方法,为蒲公英其他方面的研究奠定基础.
Abstract
Objective:In this study,the determination method of chlorogenic acid,caffeic acid,galuteolin,cichoric acid and luteolin in Pugongying(Taraxacum mongolicum)was established by HPLC,and the quality control method of Pugongying was improved and promoted.Methods:The samples of Pugongying herb were extracted by ultrasonic method,and then separated and detected by Waters ACQUITY BEH C18 chromatographic column(2.1 mm× 100 mm,1.8 μm).Acetonitrile(B)and 0.4％phosphoric acid aqueous solution(A)were used as mobile phase with gradient elution.The detection wavelength was 320 nm.Flow rate:1.0 mL/min;Injection volume:20 μL.Column temperature:35 ℃.The methodology was validated by exclusive inspection,linear relationship test,precision test,repeatability test,stability test and recovery test.Results:The concentrations of chlorogenic acid,caffeic acid,galuteolin,cichoric acid and luteolin ranged from 60 to 1 920 μg/mL,3.22 to 103 μg/mL,3.22 to 103 μg/mL,68.125 to 2 180 μg/mL and 3.31 to 106 μg/mL,respectively.Linear regression equation was carried out with the concentration(X)of each component as the abscissa and the peak area(y)as the ordinate,showing a good linear relationship(r＞0.998 7),and the av-erage recovery was 98.62％-101.43％.The RSD range was 0.68％-1.86％.The instrument precision,method repeatability and method stability were good(RSD＜2％).Conclusion:The method has good separation,precision,stability,repeatability and high recovery rate.It can be used as a quality control method of Pugongying and lays a foundation for other research of Pugongying.
基金项目
齐齐哈尔市社会发展科技攻关计划(LSFGG-2022048)
维普
万方数据