Determination of five components in Pugongying by HPLC
Objective:In this study,the determination method of chlorogenic acid,caffeic acid,galuteolin,cichoric acid and luteolin in Pugongying(Taraxacum mongolicum)was established by HPLC,and the quality control method of Pugongying was improved and promoted.Methods:The samples of Pugongying herb were extracted by ultrasonic method,and then separated and detected by Waters ACQUITY BEH C18 chromatographic column(2.1 mm× 100 mm,1.8 μm).Acetonitrile(B)and 0.4％phosphoric acid aqueous solution(A)were used as mobile phase with gradient elution.The detection wavelength was 320 nm.Flow rate:1.0 mL/min;Injection volume:20 μL.Column temperature:35 ℃.The methodology was validated by exclusive inspection,linear relationship test,precision test,repeatability test,stability test and recovery test.Results:The concentrations of chlorogenic acid,caffeic acid,galuteolin,cichoric acid and luteolin ranged from 60 to 1 920 μg/mL,3.22 to 103 μg/mL,3.22 to 103 μg/mL,68.125 to 2 180 μg/mL and 3.31 to 106 μg/mL,respectively.Linear regression equation was carried out with the concentration(X)of each component as the abscissa and the peak area(y)as the ordinate,showing a good linear relationship(r＞0.998 7),and the av-erage recovery was 98.62％-101.43％.The RSD range was 0.68％-1.86％.The instrument precision,method repeatability and method stability were good(RSD＜2％).Conclusion:The method has good separation,precision,stability,repeatability and high recovery rate.It can be used as a quality control method of Pugongying and lays a foundation for other research of Pugongying.
万方数据
维普
