Efficacy of Taoren Xikang Wan on osteoarthritis-related cytokines and chondrocyte apoptosis
Objective:The effect and mechanism of Taoren Xikang Wan(桃仁膝康丸)in the treatment of osteoarthritis by cytological methods were explored.Methods:Human synovial fibroblasts were induced by TNF-α to establish an acute inflammatory model,a M1 polarization model of human macrophages were induced to established by LPS,and human chondrocytes were induced by CoCl2 to establish a hypoxic apoptosis model.Detection of cell toxicity of Taoren Xikang Wan was performed by CCK-8 method,and the expressions of Full MMP9,Cleaved MMP9,TNF-α,IL-6,Full Caspase-3 and Cleaved Caspase-3 were detected by Western Blot.Results:Taoren Xikang Wan concentration of 1 μL/mL and below had no effect on the viability of cells.Western Blot results showed that Taoren Xikang Wan significantly inhibited the generation and excitation of MMP9 in synovial fibroblasts,the generation of inflammatory factors in M1 polarization of macrophages,and the excitation of Caspase-3 in chondrocytes.Conclusion:Taoren Xikang Wan show therapeutic effects on osteoarthritis by inhibiting the generation and excitation of degrading enzyme,generation of inflammatory cytokines and chondrocyte apoptosis.Its mechanism involves degradation of cartilage matrix,inflammatory biological process and cell apoptosis pathway,which verifies the results of network pharmacology analysis.
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