Network pharmacological analysis of treating influenza with Fuzheng Gankang Wan
Objective:To investigate the active ingredients and mechanism of action of Fuzheng Gankang Wan(扶正感康丸)in the treatment of H1N1 influenza and influenza B virus based on network pharmacology.Methods:The effective components and action targets of Fuzheng Gankang Wan were obtained through the database of TCMSP.H1N1 influenza and influenza B virus disease genes were searched by GeneCards and NCBI databases.The common target of active ingredient-disease,and the data of protein-protein interaction network were obtained through STRING database.Then Cytoscape software was used to draw the network diagram and filter the core targets.After Metascape database was used to gain enrichment analysis on core targets to obtain pathway key targets.Through BioGPS,DisGeNET and GeneCards websites,relevant information on tissue localization and subcellular distribution of key targets was obtained.Results:A total of 85 active components of 6 main drugs of Fuzheng Gankang Wan were obtained,and 41 targets of intersection with H1N1 influenza,and 106 targets with influenza B virus.Quercetin,kaempferol are the core components.TNF,IL-6,etc.are the key targets of H1N1 influenza.AKT1 and IL-6 and so on are the key targets of influenza B virus.GO enrichment analysis showed that Fuzheng Gankang Wan were mainly involved in biological processes such as positive regulation of cell death and negative regulation of cell differentiation,cellular components such as membrane raft,membrane side,and molecular functions such as RNA polymerase Ⅱ specific DNA binding transcription factor binding,protein homodimerization activity.The results of KEGG analysis showed that Fuzheng Gankang Wan were mainly involved in tumor signal pathway,AGE-RAGE signal pathway in diabetic complications and small cell lung cancer in the treatment of H1N1 influenza,while Fuzheng Gankang Wan were mainly involved in tumor signal pathway,PI3K-Akt signal pathway,nuclear factor-κB signal pathway and so on in the treatment of influenza B virus.According to the target distribution of Fuzheng Gankang Wan in the treatment of H1N1 influenza and influenza B virus,it is speculated that immune cells play an important role in improving immune function and treating these diseases.Conclusion:Fuzheng Gankang Wan can regulate AKT1,TNF,MMP9,IL-10,IL-6 and other disease targets of NF-κB,PI3K-Akt and other signal pathways to interfere with biological process to treat influenza.
NETL
NSTL
万方数据
维普
