The network pharmacological mechanism of Lianqiao in treating acne
Objective:To investigate the mechanism of action of Lianqiao(Fructus Forsythiae)in the treatment of acne by network pharmacology and molecular docking techniques.Methods:Relevant acne targets were collected from GeneCards,OMIM and NCBI databases,and the active ingredients of Lianqiao were retrieved through the Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform(TCMSP).The Swiss Target Prediction database was used to obtain the targets of the active ingredients of Lianqiao.The intersection of acne-related targets with the targets of active ingredients of Lianqiao was taken as the potential targets for the treatment of acne.The protein-protein interaction network of the targets was constructed using the STRING database,and the results were imported into Cytoscape 3.7.1 software for topological analysis to extract the core targets.GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on the core targets using the DAVID database to construct the active ingredient-core target-pathway network,and topology analysis was performed to screen the core ingredients and core targets.The interactions of core components and core targets were verified by molecular docking using AutoDock Vina.Results:A total of 19 active ingredients of Lianqiao were obtained,corresponding to 509 targets,1 561 disease targets,and 89 intersection targets.After protein-protein interaction and topological analyses,16 core targets were obtained,and the core targets involved 681 gene function entries and 160 pathways(P<0.05).The network of active ingredient-core target-pathway was topologically analyzed,and 16 active ingredients including quercetin,lignan,baicalein and kaempferol,and 16 key targets including PTGS2,RELA,TNF,PPARG,IL-6,JUN and CXCL8 were screened out.Conclusion:Lianqiao shows the characteristics of multi-components,multi-targets and multi-pathways in the treatment of acne,and the active ingredients of Lianqiao may regulate the expression of PTGS2,RELA,TNF,PPARG,IL-6,JUN,CXCL8 and other proteins to achieve the therapeutic effect.
LianqiaoAcneNetwork pharmacologyMolecular dockingMechanism of action
NETL
NSTL
万方数据
维普
