A study on the action mechanism of long snake moxibustion in the treatment of ankylosing spondylitis based on fecal metabolomics
Objective:To observe the effect of long snake moxibustion on fecal metabolites of patients with ankylosing spondylitis(AS)based on metabolomics approach,and to explore its mechanism in the treatment of AS.Methods:A total of 30 AS patients and 30 healthy volunteers were recruited,and the AS patients were treated by long snake moxibustion once a week for 12 weeks.The levels of serum ESR and hs-CRP in patients with AS before and after treatment were measured.UPLC-Q-TOF/MS detection technology was used to analyze the fecal samples of patients with AS before and after treatment and healthy people.PCA and OPLS-DA were used to screen the differential metabolites,and the MetaboAnalyst website was used to analyze the relevant metabolic pathways.Results:Serum ESR and hs-CRP levels of AS patients received long snake moxibustion treatment were lower than before treatment(P<0.05).Fecal metabolites of healthy volunteers and AS patients before treatment,and AS patients before treatment and after treatment can be clearly distinguished.A total of 36 different metabolites have been identified,and their metabolic pathway involves riboflavin metabolism,sphingolipids metabolism,histidine metabolism,β-Alanine metabolism,citric acid cycle,and pyruvate metabolism.Conclusion:Long snake moxibustion can reduce inflammation in AS patients.Its treatment is related to regulating the level of intestinal metabolites.The mechanism involves riboflavin metabolism,sphingolipids metabolism,amino acid metabolism,citric acid cycle and other aspects.
Long snake moxibustionAnkylosing spondylitisFecal metabolomics
NETL
NSTL
万方数据
维普
