The effects of Tongluo Huoxue San on patella of rats with papain-induced inflammation
Objective:To observe the effects of Tongluo Huoxue San(通络活血散)on patella of rats with papain-induced inflammation.Methods:Rat models of patellar malacia induced by papain were used to investigate the changes of body temperature,patellar surface morphology,patellar tissue,and TNF-α and TGF-β1 in synovium by infrared thermal imaging,microscopy,and immunohistochemical techniques.Results:① Infrared thermal imaging showed that the high and medium dose groups of Tongluo Huoxue San had the deepest red color,followed by the blank group,the diclofenac diethylamine emulsion group,and the low dose group of Tongluo Huoxue San,and the red color in the model group was the lightest.② Metallographic microscope observation showed that the patella of the rats in the blank group had a smooth surface and even arranged uniformly.The patella surface of the rats in the model group was rough,with multiple fissures and irregular arrangement.The patella surface of rats was rough,with many small cracks and irregular arrangement in the diclofenac diethylamine emulsion group.The surface of the patella of rats was smooth,uneven,sparse,uneven arrangement,and a few small cracks in the high,medium and low dose groups of Tongluo Huoxue San.③ The synovial tissue surface of the rats in the blank group was covered with a single layer of synovial cells,and the patella tissue was clearly stratified.Synovial hyperplasia,chronic inflammation,irregular patella surface,pannus formation,degeneration of surface reserve layer cells,obvious proliferation of proliferative layer cells,and disordered layered structure were observed in the model group.The synovial surface of rats in the high and medium dose groups of Tongluo Huoxue San was covered with a single layer of synovial cells with slight hyperplasia.The cells of the reserve layer on the patellar surface were proliferated,and the stratification was basically clear.The synovial tissue of the rats in the low-dose group of Tongluo Huoxue San and the diclofenac diethylamine emulsion group was hyperplasia with a small amount of inflammatory cells infiltrated,the reserve layer cells of patella surface were locally degraded,and the proliferative layer cells showed obvious hyperplasia.④ In terms of the positive rate of TNF-α immunohistochemical staining,the model group,the diclofenac diethylamine emulsion group,and the high,medium and low dose groups of Tongluo Huoxue San were all higher than the blank group(P＜0.05).The model group was higher than the diclofenac diethylamine emulsion group,and the high,medium and low dose groups of Tongluo Huoxue San(P＜0.05).The low dose group of Tongluo Huoxue San was higher than the diclofenac diethylamine emulsion group,and the medium and high dose groups of Tongluo Huoxue San(P＜0.05).There was no significant difference among the diclofenac diethylamine emulsion group,the middle and high dose groups of Tongluo Huoxue San(P＞0.05).In term of the positive rate of TGF-β 1 immunohistochemical staining,the model group,the diclofenac diethylamine emulsion group,and the high,middle and low dose groups of Tongluo Huoxue San were all higher than the blank group(P＜0.05).The model group was lower than the diclofenac diethylamine emulsion group,and the high,middle and low dose groups of Tongluo Huoxue San(P＜0.05).The diclofenac diethylamine emulsion group was lower than the middle and high dose groups of Tongluo Huoxue San(P＜0.05),and there was no significant difference between the diclofenac diethylamine emulsion group and the low dose group of Tongluo Huoxue San(P＞0.05).The low dose group of Tongluo Huoxue San was lower than the middle and high dose groups of Tongluo Huoxue San(P＜0.05),and there was no statistically significant difference between the middle and high dose groups of Tongluo Huoxue San(P＞0.05).Conclusion:Tongluo Huoxue San can promote the blood circulation in rats,significantly reduce the inflammatory response caused by papain,and promote the repair and regeneration of patella surface cells and tissues in rats.
万方数据
维普
