A study on the mechanism of Suhuang Xiezhuo Wan in the treatment of renal interstitial fibrosis and regulation of ferroptosis based on network pharmacology
Objective:To investigate the mechanism of action of Suhuang Xiezhuo Wan(苏黄泻浊丸)in the treatment of renal interstitial fibrosis(RIF)and regulation of ferroptosis based on network pharmacology.Methods:The TCMSP,STRING,OMIM,Gene Cards and DisGeNet were used to screen the active ingredients and RIF targets of Suhuang Xiezhuo Wan.The active ingredient of Suhuang Xiezhuo Wan-RIF target network was constructed by Cytoscape 3.6.0 software.The interaction network between the prescription and the RIF targets was constructed by STRING database.The enrichment analysis of KEGG was carried out by DAVID database.FerrDb database was used to obtain the ferroptosis-regulating genes,and finally the common target genes were obtained to construct the active ingredient of Suhuang Xiezhuo Wan-RIF and ferroptosis genes network.STRING database was used to construct the common target protein-protein interaction network,and KEGG analysis was carried out by the DAVID database.Results:A total of 102 active ingredients and 177 RIF-associated target genes were screened.After GO and KEGG analyses,the biological process of Suhuang Xiezhuo Wan in the treatment of RIF was related to the positive regulation of RNA polymerase II to promote transcription,the activity of transcription factors and sequence-specific DNA binding and so on,and it could play roles in the regulation of the lipids and atherosclerosis,the IL-17 signaling pathway,the TNF signaling pathway,the PI3K-Akt signaling pathway,the Th17 cell differentiation,and cellular senescence.There are 34 common genes regulating ferroptosis,which may be involved in IL-17 signalling pathway,HIF-1 signalling pathway,ErbB signalling pathway,lipid metabolism,Th17 cell differentiation,C-type lectin receptor signalling pathway,and TNF signalling pathway.Conclusion:This study predicted the main compounds,targets and pathways of Suhuang Xiezhuo Wan on RIF and its regulation of ferroptosis,providing theoretical references for the subsequent in-depth study of the mechanism of action of Suhuang Xiezhuo Wan on RIF.
Network pharmacologyRenal interstitial fibrosisFerroptosisMechanism of actionSuhuang Xiezhuo Wan
万方数据
维普
