Discussion on the mechanism of Kuiyuling ointment in the treatment of diabetic foot based on network pharmacology
Objective:To explore the possible mechanism of Kuiyuling ointment(清愈灵膏)in the treatment of diabetic foot based on network pharmacology.Methods:Based on the TCMSP and HERB databases,the active ingredients and targets of Kuiyuling ointment were obtained,and the disease-related targets of diabetic foot were collected through GeneCards and OMIM databases.Point-to-point mapping,the obtained intersection target is the potential target of Kuiyuling ointment in the treatment of diabetic foot.CytoScape 3.8.0 software was used to construct an active ingredient-intersection target network,and a PPI network was constructed through the STRING database.Targets were subjected to GO function and KEGG pathway enrichment analysis.Results:A total of 99 active ingredients and 364 potential targets were screened,and core active ingredients such as quercetin,sandapic acid,isopimaric acid,asiatic acid and kaempferol were obtained,core active ingredients such as MAPK1,AKTl,EGFR,ESR1 were obtained.A total of 170 signaling pathways were screened,and key pathways such as PI3K-Akt signaling pathway,MAPK signaling pathway,and AGE-RAGE signaling pathway were obtained.Conclusion:Kuiyuling ointment may act on potential core targets such as MAPK1,AKT1,EGFR,ESR1 through active ingredients such as quercetin,sanda-pimamate,and isopimaric acid,and regulate key pathways such as PI3K-Akt signaling pathway,MAPK signaling pathway,AGE-RAGE signaling pathway in the treatment of diabetic foot.
Kuiyuling ointmentDiabetic footNetwork pharmacologyMechanism of action
NETL
NSTL
万方数据
