Effects of the Dachengqi decoction on gastrointestinal function and inflammatory factors in rats with sepsis gastrointestinal dysfunction
Objective:To study the effects of the Dachengqi decoction(大承气汤)on gastrointestinal function and inflammatory factors in rats with septic gastrointestinal dysfunction.Methods:Fifty male Wistar rats were randomly divided into the sham-operated group,model group,the Dachengqi decoction high(10 g/kg),medium(5 g/kg)and low(2.5 g/kg)dose groups,with ten rats in each group.Except for sham-operated group,the rest were given cecal ligation and perforation to estabish septic gastrointestinal dysfunction models.The Dachenqi decoction groups were given corresponding dose of the Dachenqi decoction,the sham-operated group and the model group were given the equal amounts of physiological saline,were continuously gavaged once a day,for 7 days.After administration,the gastric residual rate and intestinal advancement rate of rats were measured by semi-solid paste method,and the serum levels of gastrointestinal hormones motilin,gastrin and inflammatory factors tumor necrosis factor-α and interleukin-1β were detected by enzyme-linked immunosorbent assay.Results:Compared with the sham-operated group,gastric emptying rate,intestinal propulsion rate,motilin and gastrin levels in the model group were significantly decreased,while tumor necrosis factor-α and interleukin-1β levels were significantly increased,with significant statistical differences(P＜0.01).Compared with the model group,the gastric emptying rate and intestinal propulsion rate of rats in each dose groups of the Dachengqi decoction were increased to varying degrees,with significant statistical differences(P＜0.01);the levels of motilin and gastrin in the high-dose and medium-dose groups of the Dachengqi decoction were significantly increased,with significant statistical differences(P＜0.01).The level of tumor necrosis factor-α in each dose groups of the Dachengqi decoction were significantly decreased,and the differences were statistically significant(P＜0.01),while the level of interleukin-1β in the high-dose and medium-dose groups of Dachengqi decoction were significantly decreased,and the differences were statistically significant(P＜0.01).Conclusion:Dachengqi decoction could effectively promote gastrointestinal motility in rats with septic gastrointestinal dysfunction,increase the secretion level of gastrointestinal hormones,inhibit the release of inflammatory factors,and reduce the inflammatory reaction of sepsis.
The Dachengqi decoctionSepsisGastrointestinal dysfunctionInflammatory factor
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