The neuroprotective effect of isorhapontigenin on cerebral ischemia/reperfusion injury in rats by regulating PI3K/Akt signaling pathway
Objective:To investigate the effect and action mechanism of isorhapontigenin(ISO)on ischemic penumbra neurons injury in ischemia-reperfusion rats by regulating PI3K/Akt signaling pathway.Methods:A total of 60 male SD rats were randomly divided into the sham operation group,the model group and the ISO group,with 20 rats in each group.The sham operation group was given operation but no thread.The model group was given thread embolism method to establish cerebral ischemia-reperfusion injury model.The ISO group was injected ISO 2 hours after reperfusion.Neurological impairment was assessed by Z-Longa.Cerebral infarction rate was measured by TTC staining.Brain tissue and neuron injury were observed by HE staining and Nissl staining.IL-6,IL-1β,tumor necrosis factor-α(TNF-α),superoxide dismutase(SOD)and malondialdehyde(MDA)content were detected by ELISA.The levels of p-Akt and p-PI3K were detected by Western blotting.Results:The nerve function injury degree,cerebral infarction rate and inflammatory factor level of rats in the ISO group were significantly lower than those in the model group(P＜0.05),the level of SOD in the ISO group was significantly higher than that in the model group(P＜0.05).The brain tissue in the ISO group had lower inflammatory cell infiltration and more complete neuron structure compared with the model group.The protein expressions of p-Akt and PI3K in the model group were decreased significantly,and the protein expressions of p-Akt and PI3K in the ISO group were increased significantly compared with the model group(P＜0.05).Conclusion:ISO may clear oxygen free radicals and reduce inflammation by activating PI3K/Akt signaling pathway to reduce brain cell injury caused by cerebral ischemia-reperfusion.
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维普
