Network pharmacology research of treating diabetic retinopathy with Sangye-Juhua
Objective:To predict the active ingredients,targets of action and molecular mechanisms of Sangye(Folium Mori)-Juhua(Flos Chrysanthemi)for the treatment of diabetic retinopathy using a network pharmacology study.Methods:In TCMSP database,Sangye and Juhua were used as search objects to screen out their active ingredients and targets;then in GeneCards and OMIM database,"Diabetic retionpathy"was used as keywords to find the relevant targets of the disease.Cytoscape was used to build the target relationship network,and STRING platform was used to build the protein-protein interaction network.R language software was applied to enrich the GO function analysis and KEGG pathway analysis of common targets to obtain the biological functions and signaling pathways of the core genes of Sangye-Juhua for DR treatment.Molecular docking of the screened core active ingredients of Sangye-Juhuu with the core disease targets was performed by PubChem,AutoDock Tools,Vina and PyMol.Results:A total of 117 active ingredients and 1 293 predicted targets of Sangye-Juhua were obtained by screening,with 438 genes related to DR,294 targets of Sangye-Juhua action,and 72 targets of Sangye-Juhua action on DR.Core targets of PPI network were CCND1,JUN,EGFR,ERBB2,NOS2,EGF,TNF,NFKBIA,MYC,CTNNB1.The GO functional enrichment analysis yielded 1 927 biological processes,89 molecular functions and 58 cellular compositions.A total of 134 signaling pathways were analyzed by KEGG pathway enrichment.Conclusion:Sangye-Juhua mainly acts on target genes such as JUN,NFKBIA,MYC,TNF and CTNNBl through active ingredients such as artemisinin,naringenin,moraxin,spermidine and albanol,and through biological processes such as response to oxidative stress,regulation of apoptotic signaling pathway,response of lipopolysaccharide,and cellular response to chemical stress,by taking lipid-atherosclerosis signaling pathway,fluid shear stress-atherosclerosis signaling pathway as the main pathways to play a therapeutic role in treating DR.
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