A study on the mechanism of action of Chuanxiong-Chishao in the treatment of angina pectoris in coronary artery disease based on network pharmacology
Objective:To investigate the mechanism of action of Chuanxiong(Rhizoma Chuanxiong)and Chishao(Radix Paeoniae Rubra)in the treatment of angina pectoris in coronary heart disease(CHD-AP)through the concept and technology of network pharmacology.Methods:The TCMSP database was used to retrieve the main active ingredients and targets of Chuanxiong and Chishao,and then the GeneCards,OMIM and TTD databases were used to obtain the relevant target information of CHD-AP,followed by the intersection targets of Chuanxiong-Chishao and CHD-AP,and the Cytoscape 3.10.0 software was used to construct the network of Chinese medicine-ingredient-target-disease target.The intersection target information was then used to construct a protein-protein interaction(PPI)network by the STRING database to screen out the core targets.The intersection targets were enriched for GO function and KEGG pathway using the David database.Results:A total of 36 major active ingredients were screened from Chuanxiong and Chishao,including 7 from Chuanxiong and 29 from Chishao,and a total of 166 relevant action targets were obtained,after screening and de-weighting,there were 76 from CHD-AP,1 213 from CHD-AP,35 from Chuanxiong and Chishao and CHD-AP,with 18 core targets.The results of GO functional enrichment analysis showed that they mainly involved in biological processes such as response to exogenous stimuli,negative regulation of apoptotic process,extracellular regions,plasma membrane,enzyme binding,identical protein binding,etc..The results of KEGG pathway enrichment analysis showed that there were mainly signaling pathways such as cancer pathway,fluid shear and atherosclerosis,Kaposi's sarcoma-associated herpesvirus infection,proteoglycans in cancer,chemical carcinogenesis-receptor activation,lipids and atherosclerosis,etc..Conclusion:This study initially revealed the main active ingredients,targets,biological processes and pathways of Chuanxiong-Chishao in the treatment of CHD-AP,which provides a reference basis for further research on its pharmacological effects and its clinical application.
Network pharmacologyChuanxiongChishaoAngina pectoris in coronary angina pectorisMechanism of action
NETL
NSTL
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