An experimental study on homing effects of the Buyang Huanwu decoction on transplanted BMSCs in rats with acute myocardial infarction
Objective:To observe the effect of the Buyang Huanwu decoction(补阳还五汤)on homing of transplanted bone mesenchymal stem cells(BMSCs)in rats with acute myocardial infarction and the protective effect of the combination of the two on myocardial injury.Methods:A total of 48 SPF Sprague-Dawley(SD)rats with body weight(220±20)g at the age of 7 to 8 weeks were selected.They were randomly divided into 4 groups:blank group,model group,BMSCs transplantation group,Buyang Huanwu decoction+BMSCs transplantation group.After 3 days of intragastric administration,6 rats in each group were randomly collected for blood serum collection.The levels of myocardial enzymes(CK-MB,cTnⅠ)were detected by biochemical method.The expression of inflammatory factor interleukin-6(IL-6)and tumor necrosis factor(TNF-α)were detected by ELISA.The homing of transplanted BMSCs was observed under microscope by taking myocardial tissue of myocardial infarction and marginal area.The cardiac function of the remaining rats was analyzed by Doppler echocardiography 4 weeks later.Results:Compared with model group,the contents of CK-MB and cTnI were decreased significantly in the Buyang Huanwu decoction+BMSCs transplantation group,and the difference was statistically significant(P＜0.01).Compared with model group and BMSCs transplantation group,the levels of IL-6 and TNF-α in the Buyang Huanwu decoction+BMSCs transplantation group were decreased,and the difference was statistically significant(P＜0.05).Fluorescence microscopy showed that the expression number of fluorescent cells in the Buyang Huanwu decoction+BMSCs transplantation group was higher than that in the BMSCs transplantation group alone(P＜0.05).Compared with the model group,left ventricular ejection fraction in the Buyang Huanwu decoction+BMSCs transplantation group was significantly increased,and the difference was statistically significant(P＜0.05).Conclusion:The Buyang Huanwu decoction can promote the homing of transplanted BMSCs and play a protective role in myocardial injury.The mechanism of the Buyang Huanwu decoction may be related to inhibiting local inflammation after myocardial infarction,removing blood stasis and generating new blood,and improving the local metabolic environment of myocardium.
Bone marrow mesenchymal stem cellAcute myocardial infarctionThe Buyang Huanwu decoction
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