Objective:To investigate the effect of hirudin on fibrinolytic function in rat with early sepsis.Methods:We randomly divided 45 healthy rat into a sham operation(SO)group,a caecal ligation puncture(CLP)group,and an hirudin(HI)group.Ten rats were randomly selected from each group for 24-hour survival observation.Blood samples and pathological tissues were collected at 6 and 12 hours after surgery.Antithrombin Ⅲ,fibrinogen,plasminogen,plasmin Inhibitor,d-dimer and fibrin degradation products were tested at 6 hours and 12 hours after operation.Results:After 24 hours of surgery,the survival rates of rats in the SO group,CLP group,and HI group were 100％,40％and 80％,respectively.The FIB was significantly increased at 12 hours after operation in the SO group as compared with that in the CLP group and HI group(P＜0.05),but that was remarkably higher in the HI group than the CLP group(P＜0.05).The AT Ⅲ was significantly decreased at 6 hours and 12 hours after operation in the CLP and HI group as compared with that in the SO group(P＜0.05),and that was remarkably lower at 12 hours after operation in the CLP group than the HI group(P＜0.05).The PLG was significantly decreased at 12 hours after operation in the CLP group and HI group than the SO group(P＜0.05).The PI was significantly decreased at 12 hours after operation in the SO and HI group than the CLP group(P＜0.05).The FDP was significantly decreased at 6 hours and 12 hours after operation in the SO and HI group than the CLP group(P＜0.05).Intestinal mucosal epithelial necrosis,interstitial edema,and inflammatory cell infiltration were significantly reduced more in the CLP group than the HI group.Conclusion:Hirudin can regulate profibrinolysis and anti-fibrinolysis and enhance the anticoagulant and anti-inflammatory effect in the early stages of sepsis,which can improve fibrinolytic function.
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