Experimental study on improving the inhibition of bacterial sepsis T cell proliferation by blocking TGF-β
Objective:To explore the effect of anti-inflammatory cytokines on the immunosuppression of T lymphocytes in lipopolysaccharide bacterial sepsis.Methods:Gram-negative bacterial component lipopolysaccharide was used to induce dendritic cells to replicate the immuno-suppressive model of bacterial sepsis.Flow cytometry was used to detect the expression of pro-inflammatory cytokines IL-1,IL-6,TNF-α and anti-inflammatory cytokines IL-10,IL-17,TGF-β of dendritic cells in the control group and the model group.Results:Compared with the control group,the positive percentages of the pro-inflammatory cytokines IL-1,IL-6,TNF-α and anti-inflammatory cytokines TGF-β of the dendritic cells in the model group were all significantly increased(P＜0.05).But there was no statistical difference in the anti-inflammatory cytokines IL-10,IL-17(P＞0.05).Compared with the model group,the T cell proliferation response of the αTGF-β+the model group was significantly increased.Conclusion:The up-regulation of pro-inflammatory cytokines IL-1,IL-6 and TNF-α in dendritic cells may be involved in mediating the pro-inflammatory response process in bacterial sepsis.Importantly TGF-β blocking can significantly reverse the inhibition of lipopolysaccharide-induced T lymphocyte proliferation in sepsis.
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维普
