A study on the mechanism of the Chaihu Guizhi decoction in the treatment of Brucella spondylitis based on network pharmacology
Objective:To study the mechanism of the Chaihu Guizhi decoction(柴胡桂枝汤)in the treatment of Brucella spondylitis by network pharmacology,so as to provide a new idea for the treatment of Brucella spondylitis with classical prescriptions.Methods:TCMSP was used to collect the main targets and compounds of 9 traditional Chinese medicines in the Chaihu Guizhi decoction,and the target genes of compounds were matched in Uniport database.Cytoscape 3.9.1 was used to construct the network graph of traditional Chinese medicine-compounds-genes.The related targets of Brucellosis were obtained from GeneCards and Drugbank databases,and the core target PPI network was constructed in the STRING database.The David database was used to perform GO functional enrichment analysis and KEGG pathway enrichment analysis,and the bioinformatics online tool was used for mapping.Finally,AutoDock and Pymol software were used to perform molecular docking and visualization of the active components with high degree value and potential targets of traditional Chinese medicine-compound-gene network graph.Results:A total of 170 major chemical components(quercetin,β-sitosterol,kaempferol,etc.)and 290 potential targets(PTGS2,HSP90AB1,PTGS1,etc.)in the Chaihu Guizhi decoction were screened.A total of 271 enrichment results were obtained by GO functional enrichment analysis,and 85 enrichment results were obtained by KEGG pathway analysis.The Chaihu Guizhi decoction was mainly involved in pertussis,lipid and atherosclerosis,IL-17 signaling pathway,NF-κB signaling pathway and other signaling pathways.It was closely related to infectious diseases,immune response and immune disorders,oxidative stress,and inflammatory response.Conclusion:This study preliminarily shows that the Chaihu Guizhi decoction has multi-components,multi-targets and multi-pathways in the treatment of Brucella spondylitis.
Brucella spondylitisThe Chaihu Guizhi decoctionNetwork pharmacologyMechanism of action
万方数据
维普
