A study on the mechanism of Huangqi-Fuling in the treatment of nephrotic syndrome based on network pharmacology and molecular docking
Objective:To investigate the mechanism of action of core Chinese medicines Huangqi(Radix Astragali)-Fuling(Poria)in the treatment of nephrotic syndrome(NS)based on network pharmacology and molecular docking technology.Method:The active ingredients of Huangqi-Fuling and their target proteins were collected from three databases:TCMSP,UniProt and Herb.Targets of action related to NS were collected in the OMIM and GeneCards database.The target proteins of Huangqi-Fuling and NS were taken to be intersected,then the Venny diagram was drawn,and further their relationship networks were constructed in Cytoscape software.The PPI network was constructed by the STRING,and the enrichment analyses of GO and KEGG pathway were performed using R language.Finally,AutoDock software was used for molecular docking verification,and Pymol was used to visualize the results.Results:A total of 34 active ingredients in Huangqi-Fuling met the initial screening requirements,and 96 intersecting targets were further identified,which were closely related to 2 140 biological processes,134 molecular functions and 71 cellular components.Potential mechanisms of action of Huangqi-Fuling were regulation of lipid and atherosclerosis,PI3K-Akt,hepatitis B,MAPK signalling pathways,etc.The Huangqi-Fuling core components such as quercetin,kaempferol,7-O-methylisomucronulatol,formononetin,isorhamnetin,and hederagenin were docked to the core target proteins of adenylate kinase isoenzyme 1,TNF,IL-6,PTGS2 and IL-1β with excellent binding.Conclusion:Huangqi-Fuling has the advantage of diversity of components,targets and pathways,and mainly mediates inflammatory response,immune response and oxidative stress response to exert therapeutic effects.This conclusion provides theoretical support for clinical practice,but more relevant experiments are still needed to verify in the later stage.
Huangqi-FulingNephrotic syndromeMechanism of actionMolecular dockingNetwork pharmacology
万方数据
维普
