The protective effect of serum containing Baiban Granules on oxidative damage of human epidermal melanocytes induced by H2O2
Objective:To study the protective effect of serum containing Baiban Granules(白斑冲剂)on the oxidative damage of normal epidermal melanocytes induced by hydrogen peroxide(H2O2).Methods:Normal epidermal melanocytes PIG1 were treated with different con-centrations of H2O2,the activity of PIG1 cells was detected by CCK-8 method,and the optimal concentration of H2O2 was selected to establish the oxidative stress model of melanocytes in vitro.Different concentrations of Baiban Granules containing serum and action time were used to interfere with melanocyte oxidative stress model.CCK-8 method was used to screen the appropriate concentration and action time of Baiban Granules.PIG1 cells at logarithmic growth stage were randomly divided into the control group(PIG1 cells+normal rat serum),the model group(PIG1 cells+H2O2),and Bai-ban Granules group(PIG1 cells+Baiban Granules drug-containing serum+H2O2).The protective effect of Baiban Granule on oxidative damage of PIG1 cells was observed under inverted microscope after intervention.PIG1 cell apoptosis was detected by flow cytometry.Results:The activity of PIG1 cells was decreased in a concentration-dependent manner under the effect of H2O2.The oxidative stress model of PIG1 cells was established by 0.8 mmol/L H2O2 for 24 h according to ID50 value.Under oxidative stress,PIG1 cells were treated with different concentrations of drug-containing serum for 0 h,12 h,24 h,48 h and 72 h.Compared with the model group,20％drug-containing senim showed the most significant increase in cell viability for 24 h.Therefore,20％Baiban Granules serum was selected as the optimal concentration and action time of drug-containing serum for 24 hours.The inverted microscope showed that the cell density was decreased and the number of dead cells was increased in the model group compared with those in the control group.Compared with the model group,the degree of cell damage in Baiban Granules group was significantly reduced.Compared with the control group,the apoptosis ratio in the model group was significantly increased(P＜0.01).Compared with the model group,the proportion of apoptosis in Baiban Granules group was significantly decreased(P＜0.01).Conclusion:The serum containing Baiban Granules has an obvious protective effect on the oxidative damage of normal melanocytes induced by H2O2.
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