Application of pediatric massage therapy in children with spleen deficiency and its regulatory effects on intestinal flora in 60 cases
Objective:To observe the application of pediatric massage therapy in children with spleen deficiency constitution and its regulatory effect on intestinal flora.Methods:A total of 60 children with spleen deficiency who were treated from December 2021 to March 2023 were randomly divided into the observation group and the control group,with 30 cases in each group for comparison.The control group was instructed on lifestyle habits such as daily routines,diet,and living habits,followed up for 2 months,and underwent syndrome score evaluation.Stool samples were taken for intestinal flora analysis,and symptomatic treatment was given to children with various diseases during the period.The observation group received pediatric massage therapy on the basis of the control group.The intestinal flora in stool samples,syndrome score,and efficacy of treatment before and after treatment were observed in both groups of children;16SrDNA amplification sub-experiments were conducted and analyzed for intestinal flora.Results:Both groups showed good therapeutic effects after treatment,with improvements in syndrome scores(P＜0.05).In the observation group,the level of beneficial bacteria Rothia in the gut microbiota significantly increased(P＜0.05),while harmful bacteria Escherichia significantly decreased(P＜0.05).After treatment,there were statistically significant differences in Rothia and Escherichia levels between the control group and the observation group;the observation group showed better therapeutic effects.There was no statistically significant difference in the effective rate between the two groups after treatment(P＞0.05).A comparison of LEfSe LDA of genus-level microbial community diversity before and after treatment in the two groups revealed that the genera of Alculigenes foecalis genus and Rothia significantly genus increased in the control group after treatment(P＜0.05).Compared with before treatment,there was no statistically significant difference in Escherichia after treatment(P＞0.05),and it was not marked.A comparison of the differences in key genera levels between the two groups before and after treatment revealed that there were no statistically significant differences in the genus of Alculigenes foecalis,Escherichia,and Rothia between the two groups before treatment(P＞0.05).After treatment,the levels of Alculigenes foecalis genus and Rothia genus in the control group significantly increased(P＜0.05),while there was no statistically significant difference in Escherichia(P＞0.05).In the observation group,the levels of Alculigenes foecalis genus and Rothia genus significantly increased(P＜0.05)before and after treatment,while the level of Escherichia significantly decreased(P＜0.05).After treatment,there was no statistically significant difference in the Alculigenes foecalis genus between the two groups(P＞0.05).However,the observation group showed a significant increase in the genus of Rothia(P＜0.05)and a significant decrease in the genus of Escherichia(P＜0.05).The Alpha diversity before and after treatment in both groups showed that there was a statistically significant difference in the Coverage index in the control group before and after treatment(P＜0.05).The differences in Shannon index,Simpson index,and Coverage index before and after treatment in the observation group were statistically significant(P＜0.05).Conclusion:Massage can regulate the gut microbiota status,increase the abundance of beneficial bacteria such as Alculigenes foecalis and Rothia,and affect human functions through the bidirectional regulation of the gut-brain axis,thereby enhancing immunity.Therefore,it is speculated that this may be one of the mechanisms of massage in regulating children's physical constitution.
Pediatric massageSpleen deficiency in childrenChildren's constitutionIntestinal flora
万方数据
维普
