Analysis of gene expression profiles related to NETs in children with RSV infection and prediction of Chinese medicine
Objective:To investigate the expression patterns of NETs-related genes in whole blood of RSV-infected children by bioinformatics methods and to predict potential Chinese medicines against NETs during RSV infection.Methods:According to the expression of NETs related genes,unsupervised cluster analysis was performed on the dataset,GSEA enrichment analysis and immune infiltration analysis were performed among the groups,and NETsDEGs among the groups were identified by difference analysis.CTD database and Coremine Medical database were used for TCM prediction.Results:According to the expression level of NETs related genes,RSV infected children were divided into two groups.Multiple pathways obtained by GSEA enrichment analysis were associated with immunity,and immunoinfiltration analysis showed that there were differences in the abundance of immune cell infiltration between the two groups,which further suggested the influence of NETs level on the immune environment of the body.MPO,AZU1,CTSG and ELANE were identified as NETsDEGs between the two groups by differential analysis,and a total of 46 related compounds were mapped accordingly,and 145 predicted Chinese medicines were obtained.The common predicted Chinese medicines of the two groups were analyzed,and it was found that the disease sites of NETs in RSV infection were mainly in the lung and spleen.It was predicted that the property and taste of traditional Chinese medicine was mainly bitter,sweet,pungent and cold,indicating that NETs might be related to wind evil in RSV infection.In addition,the two groups of corresponding predicted Chinese medicines were compared,and it was found that the expression level of NETs was related to the type and location of RSV infection,and its increase may be related to heat syndrome.Conclusion:In this study,two expressions of NETs-related genes in RSV-infected children are found through bioinformatics methods,and related Chinese medicines are predicted accordingly.Through the analysis of potential Chinese medicines,the pathogenic mechanism of RSV infection in children is enriched,and the connotation of NETs in Chinese medicine is analyzed,providing a new target for the treatment of RSV infection.
Respiratory syncytial virusNeutrophil extracellular trapping netBioinformaticsTraditional Chinese medicine prediction
万方数据
维普
